-Methyladenosine (mA) is one of the most abundant modifications of eukaryotic mRNA, but its comprehensive biological functionality remains further exploration. In this study, we identified and characterized a new flowering-promoting gene, (), in rice. encodes an RNA recognition motif (RRM)-containing RNA binding protein that is localized in the non-membranous cytoplasm ribonucleoprotein (RNP) granules and can bind both mA-modified RNA and unmodified RNA indiscriminately. We found that EHD6 can physically interact with YTH07, a YTH (YT521-B homology) domain-containing mA reader. We showed that their interaction enhances the binding of an mA-modified RNA and triggers relocation of a portion of YTH07 from the cytoplasm into RNP granules through phase-separated condensation. Within these condensates, the mRNA of a rice flowering repressor, (), becomes sequestered, leading to a reduction in its protein abundance and thus accelerated flowering through the pathway. Taken together, these results not only shed new light on the molecular mechanism of efficient mA recognition by the collaboration between an RNA binding protein and YTH family mA reader, but also uncover the potential for mA-mediated translation regulation through phase-separated ribonucleoprotein condensation in rice.

中文翻译：

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RNA结合蛋白EHD6招募m6A阅读器YTH07并将OsCOL4 mRNA隔离成相分离核糖核蛋白凝聚物以促进水稻开花


-甲基腺苷（mA）是真核mRNA最丰富的修饰之一，但其综合生物学功能仍有待进一步探索。在这项研究中，我们在水稻中鉴定并鉴定了一种新的促花基因（）。编码包含RNA识别基序（RRM）的RNA结合蛋白，该蛋白位于非膜细胞质核糖核蛋白（RNP）颗粒中，并且可以不加区别地结合m6A修饰的RNA和未修饰的RNA。我们发现 EHD6 可以与 YTH07（一种包含 YTH（YT521-B 同源性）结构域的 mA 阅读器）物理相互作用。我们发现它们的相互作用增强了 m6A 修饰的 RNA 的结合，并通过相分离缩合触发 YTH07 的一部分从细胞质重新定位到 RNP 颗粒中。在这些凝结物中，水稻开花抑制子 () 的 mRNA 被隔离，导致其蛋白质丰度减少，从而通过该途径加速开花。总而言之，这些结果不仅为通过 RNA 结合蛋白和 YTH 家族 mA 阅读器之间的协作来有效识别 mA 的分子机制提供了新的线索，而且还揭示了通过相分离的核糖核蛋白缩合进行 mA 介导的翻译调节的潜力。米。