Critical review of single-cell mechanotyping approaches for biomedical applications,Lab on a Chip

当前位置： X-MOL 学术 › Lab Chip › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Critical review of single-cell mechanotyping approaches for biomedical applications
Lab on a Chip ( IF 6.1 ) Pub Date : 2024-05-28 , DOI: 10.1039/d3lc00978e
Max Chapman ₁ , Vijay Rajagopal ₁ , Alastair Stewart _{2,

3} , David J Collins _{1,

4}

Affiliation

Accurate mechanical measurements of cells has the potential to improve diagnostics, therapeutics and advance understanding of disease mechanisms, where high-resolution mechanical information can be measured by deforming individual cells. Here we evaluate recently developed techniques for measuring cell-scale stiffness properties; while many such techniques have been developed, much of the work examining single-cell stiffness is impacted by difficulties in standardization and comparability, giving rise to large variations in reported mechanical moduli. We highlight the role of underlying mechanical theories driving this variability, and note opportunities to develop novel mechanotyping devices and theoretical models that facilitate convenient and accurate mechanical characterisation. Moreover, many high-throughput approaches are confounded by factors including cell size, surface friction, natural population heterogeneity and convolution of elastic and viscous contributions to cell deformability. We nevertheless identify key approaches based on deformability cytometry as a promising direction for further development, where both high-throughput and accurate single-cell resolutions can be realized.

中文翻译：

生物医学应用单细胞机械分型方法的批判性评论

细胞的精确机械测量有可能改善诊断、治疗并促进对疾病机制的理解，其中可以通过使单个细胞变形来测量高分辨率机械信息。在这里，我们评估了最近开发的用于测量细胞尺度刚度特性的技术；虽然已经开发了许多此类技术，但许多检查单细胞刚度的工作都受到标准化和可比性困难的影响，导致报告的机械模量存在较大差异。我们强调了驱动这种变化的基础机械理论的作用，并指出了开发新颖的机械分型设备和理论模型的机会，以促进方便和准确的机械表征。此外，许多高通量方法都受到细胞大小、表面摩擦、自然群体异质性以及弹性和粘性对细胞变形性贡献的卷积等因素的困扰。尽管如此，我们仍将基于可变形细胞术的关键方法确定为进一步发展的有希望的方向，其中可以实现高通量和准确的单细胞分辨率。

更新日期：2024-05-28

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南