BACKGROUND The establishment and maintenance of pregnancy depend on endometrial competence. Asherman syndrome (AS) and intrauterine adhesions (IUA), or endometrial atrophy (EA) and thin endometrium (TE), can either originate autonomously or arise as a result from conditions (i.e. endometritis or congenital hypoplasia), or medical interventions (e.g. surgeries, hormonal therapies, uterine curettage or radiotherapy). Affected patients may present an altered or inadequate endometrial lining that hinders embryo implantation and increases the risk of poor pregnancy outcomes and miscarriage. In humans, AS/IUA and EA/TE are mainly treated with surgeries or pharmacotherapy, however the reported efficacy of these therapeutic approaches remains unclear. Thus, novel regenerative techniques utilizing stem cells, growth factors, or tissue engineering have emerged to improve reproductive outcomes. OBJECTIVE AND RATIONALE This review comprehensively summarizes the methodologies and outcomes of emerging biotechnologies (cellular, acellular, and bioengineering approaches) to treat human endometrial pathologies. Regenerative therapies derived from human tissues or blood which were studied in preclinical models (in vitro and in vivo) and clinical trials are discussed. SEARCH METHODS A systematic search of full-text articles available in PubMed and Embase was conducted to identify original peer-reviewed studies published in English between January 2000 and September 2023. The search terms included: human, uterus, endometrium, Asherman syndrome, intrauterine adhesions, endometrial atrophy, thin endometrium, endometritis, congenital hypoplasia, curettage, radiotherapy, regenerative therapy, bioengineering, stem cells, vesicles, platelet-rich plasma, biomaterials, microfluidic, bioprinting, organoids, hydrogel, scaffold, sheet, miRNA, sildenafil, nitroglycerine, aspirin, growth hormone, progesterone, and estrogen. Preclinical and clinical studies on cellular, acellular, and bioengineering strategies to repair or regenerate the human endometrium were included. Additional studies were identified through manual searches. OUTCOMES From a total of 4366 records identified, 164 studies (3.8%) were included for systematic review. Due to heterogeneity in the study design and measured outcome parameters in both preclinical and clinical studies, the findings were evaluated qualitatively and quantitatively without meta-analysis. Groups using stem cell-based treatments for endometrial pathologies commonly employed mesenchymal stem cells (MSCs) derived from the human bone marrow or umbilical cord. Alternatively, acellular therapies based on platelet-rich plasma (PRP) or extracellular vesicles are gaining popularity. These are accompanied by the emergence of bioengineering strategies based on extracellular matrix (ECM)-derived hydrogels or synthetic biosimilars that sustain local delivery of cells and growth factors, reporting promising results. Combined therapies that target multiple aspects of tissue repair and regeneration remain in preclinical testing but have shown translational value. This review highlights the myriad of therapeutic material sources, administration methods, and carriers that have been tested. WIDER IMPLICATIONS Therapies that promote endometrial proliferation, vascular development, and tissue repair may help restore endometrial function and, ultimately, fertility. Based on the existing evidence, cost, accessibility, and availability of the therapies, we propose the development of triple-hit regenerative strategies, potentially combining high-yield MSCs (e.g. from bone marrow or umbilical cord) with acellular treatments (PRP), possibly integrated in ECM hydrogels. Advances in biotechnologies together with insights from preclinical models will pave the way for developing personalized treatment regimens for patients with infertility-causing endometrial disorders such as AS/IUA, EA/TE, and endometritis. REGISTRATION NUMBER https://osf.io/th8yf/

中文翻译：

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子宫内膜疾病的生物技术进步和再生疗法的演变：系统评价


背景妊娠的建立和维持取决于子宫内膜的能力。阿舍曼综合征 (AS) 和宫腔粘连 (IUA)，或子宫内膜萎缩 (EA) 和子宫内膜薄 (TE)，可以自发产生，也可以因病症（即子宫内膜炎或先天性发育不全）或医疗干预（例如手术）而产生、激素疗法、子宫刮除术或放射疗法）。受影响的患者可能会出现子宫内膜改变或不充分的情况，从而阻碍胚胎植入并增加不良妊娠结局和流产的风险。在人类中，AS/IUA 和 EA/TE 主要通过手术或药物治疗进行治疗，但这些治疗方法的报道疗效仍不清楚。因此，利用干细胞、生长因子或组织工程的新型再生技术已经出现，以改善生殖结果。目的和基本原理这篇综述全面总结了新兴生物技术（细胞、非细胞和生物工程方法）治疗人类子宫内膜病理的方法和结果。讨论了在临床前模型（体外和体内）和临床试验中研究的源自人体组织或血液的再生疗法。检索方法 对 PubMed 和 Embase 中的全文文章进行了系统检索，以确定 2000 年 1 月至 2023 年 9 月期间以英文发表的原始同行评审研究。 检索词包括：人类、子宫、子宫内膜、阿谢曼综合征、宫腔粘连、子宫内膜萎缩、子宫内膜薄、子宫内膜炎、先天性发育不全、刮宫、放射治疗、再生治疗、生物工程、干细胞、囊泡、富血小板血浆、生物材料、微流体、生物打印、类器官、水凝胶、支架、片材、miRNA、西地那非、硝酸甘油、阿司匹林、生长激素、黄体酮和雌激素。包括关于修复或再生人类子宫内膜的细胞、非细胞和生物工程策略的临床前和临床研究。通过手动搜索确定了其他研究。结果 在总共确定的 4366 份记录中，纳入了 164 项研究 (3.8%) 进行系统评价。由于临床前和临床研究中研究设计和测量结果参数的异质性，对研究结果进行定性和定量评估，无需进行荟萃分析。使用干细胞治疗子宫内膜病理的团体通常使用源自人类骨髓或脐带的间充质干细胞（MSC）。另外，基于富血小板血浆（PRP）或细胞外囊泡的无细胞疗法也越来越受欢迎。与此同时，基于细胞外基质（ECM）衍生的水凝胶或合成生物仿制药的生物工程策略的出现，可维持细胞和生长因子的局部递送，并报告了有希望的结果。针对组织修复和再生多个方面的联合疗法仍处于临床前测试中，但已显示出转化价值。这篇综述重点介绍了无数经过测试的治疗材料来源、给药方法和载体。 更广泛的影响促进子宫内膜增殖、血管发育和组织修复的治疗可能有助于恢复子宫内膜功能，并最终恢复生育能力。基于现有的证据、成本、可及性和治疗的可用性，我们建议开发三重打击再生策略，可能将高产间充质干细胞（例如来自骨髓或脐带）与脱细胞治疗（PRP）相结合，可能集成到 ECM 水凝胶中。生物技术的进步加上临床前模型的见解将为开发针对不孕引起的子宫内膜疾病（如 AS/IUA、EA/TE 和子宫内膜炎）患者的个性化治疗方案铺平道路。注册号 https://osf.io/th8yf/