Plant senescence is a highly regulated developmental program crucial for nutrient reallocation and stress adaptation in response to developmental and environmental cues. Stress-induced and age-dependent natural senescence share both overlapping and distinct molecular responses and regulatory schemes. Previously, we have utilized a carbon-deprivation (C-deprivation) senescence assay using Arabidopsis (Arabidopsis thaliana) seedlings to investigate senescence regulation. Here we conducted a comprehensive time-resolved transcriptomic analysis of Arabidopsis wild type seedlings subjected to C-deprivation treatment at multiple time points, unveiling substantial temporal changes and distinct gene expression patterns. Moreover, we identified ALTERED MERISTEM PROGRAM 1 (AMP1), encoding an endoplasmic reticulum protein, as a potential regulator of senescence based on its expression profile. By characterizing loss-of-function alleles and overexpression lines of AMP1, we confirmed its role as a negative regulator of plant senescence. Genetic analyses further revealed a synergistic interaction between AMP1 and the autophagy pathway in regulating senescence. Additionally, we discovered a functional association between AMP1 and the endosome-localized ABNORMAL SHOOT3 (ABS3)-mediated senescence pathway and positioned key senescence-promoting transcription factors downstream of AMP1. Overall, our findings shed light on the molecular intricacies of transcriptome reprogramming during C-deprivation-induced senescence and the functional interplay among endomembrane compartments in controlling plant senescence.

中文翻译：

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内质网蛋白 ALTERED MERISTEM PROGRAM 1 负调控拟南芥衰老


植物衰老是一个高度调控的发育程序，对于响应发育和环境线索的养分重新分配和应激适应至关重要。压力引起的和年龄依赖性的自然衰老具有重叠和不同的分子反应和调节方案。此前，我们利用拟南芥（Arabidopsis thaliana）幼苗进行碳剥夺（C-deprivation）衰老测定来研究衰老调控。在这里，我们对在多个时间点接受 C-剥夺处理的拟南芥野生型幼苗进行了全面的时间分辨转录组分析，揭示了显着的时间变化和不同的基因表达模式。此外，我们根据其表达谱确定了编码内质网蛋白的改变分生组织程序 1 (AMP1) 作为衰老的潜在调节因子。通过表征 AMP1 的功能丧失等位基因和过度表达系，我们证实了其作为植物衰老负调节因子的作用。遗传分析进一步揭示了 AMP1 和自噬途径在调节衰老方面的协同相互作用。此外，我们发现了 AMP1 和内体定位的异常 SHOOT3 (ABS3) 介导的衰老途径之间的功能关联，并将关键的衰老促进转录因子定位在 AMP1 的下游。总的来说，我们的研究结果揭示了 C 剥夺诱导的衰老过程中转录组重编程的分子复杂性以及内膜区室在控制植物衰老过程中的功能相互作用。