Carbon source is crucial for the cell growth and metabolism in microorganisms, and its utilization significantly affects the synthesis efficiency of target products in microbial cell factories. Compared with a single carbon source, co-utilizing carbon sources provide an alternative approach to optimize the utilization of different carbon sources for efficient biosynthesis of many chemicals with higher titer/yield/productivity. However, the efficiency of bioproduction is significantly limited by the sequential utilization of a preferred carbon source and secondary carbon sources, attributed to carbon catabolite repression (CCR). This review aimed to introduce the mechanisms of CCR and further focus on the summary of the strategies for co-utilization of carbon sources, including alleviation of CCR, engineering of the transport and metabolism of secondary carbon sources, compulsive co-utilization in single culture, co-utilization of carbon sources via co-culture, and evolutionary approaches. The findings of representative studies with a significant improvement in the bioproduction of chemicals via the co-utilization of carbon sources were discussed in this review. It suggested that by combining rational metabolic engineering and irrational evolutionary approaches, co-utilizing carbon sources can significantly contribute to the bioproduction of chemicals.

中文翻译：

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微生物碳源的共同利用用于化学品的生物生产


碳源对于微生物的细胞生长和代谢至关重要，其利用率显着影响微生物细胞工厂目标产物的合成效率。与单一碳源相比，共用碳源提供了一种替代方法来优化不同碳源的利用，从而有效生物合成具有更高滴度/产量/生产率的许多化学品。然而，由于碳分解代谢物抑制（CCR），优先碳源和次要碳源的顺序利用严重限制了生物生产的效率。本文旨在介绍CCR的机制，并进一步重点总结碳源共利用策略，包括缓解CCR、次生碳源运输和代谢工程、单一培养物中的强制共利用、通过共培养和进化方法共同利用碳源。本综述讨论了通过碳源的共同利用显着改善化学品生物生产的代表性研究的结果。它表明，通过结合合理的代谢工程和非合理的进化方法，共同利用碳源可以显着促进化学品的生物生产。