EMRE 是线粒体钙单向转运蛋白复合体的重要组成部分,Science

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EMRE 是线粒体钙单向转运蛋白复合体的重要组成部分
Science ( IF 44.7 ) Pub Date : 2013-11-14 , DOI: 10.1126/science.1242993
Yasemin Sancak ₁ , Andrew L Markhard , Toshimori Kitami , Erika Kovács-Bogdán , Kimberli J Kamer , Namrata D Udeshi , Steven A Carr , Dipayan Chaudhuri , David E Clapham , Andrew A Li , Sarah E Calvo , Olga Goldberger , Vamsi K Mootha

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EMRE 出现线粒体内的钙浓度受到严格调控并调节线粒体生理功能，包括控制新陈代谢和细胞死亡。桑卡克等人。(p. 1379，11 月 14 日在线发表) 完成了线粒体钙单向转运蛋白 (MCU) 的分子表征，MCU 是一种多组分通道，可在细胞器内集中钙。他们确定了一种称为“基本 MCU 调节剂”或 EMRE 的小蛋白质，它是完全组装的单向转运蛋白的钙转运活动所必需的。发现了参与维持线粒体钙水平的最后但必不可少的蛋白质成分。线粒体单向转运体是细胞器内膜中高度选择性的钙通道。其分子成分包括含 EF 手的钙结合蛋白线粒体钙摄取 1 (MICU1) 和 MICU2 以及成孔亚基线粒体钙单向转运蛋白 (MCU)。我们试图实现 uniporter holocomplex (uniplex) 的完整分子表征。亲和纯化的 uniplex 的定量质谱法回收了 MICU1 和 MICU2、MCU 及其旁系同源物 MCUb 和基本 MCU 调节器 (EMRE)，这是一种以前未表征的蛋白质。EMRE 是一种 10 千道尔顿的后生动物特异性蛋白质，具有单个跨膜结构域。在它不存在的情况下，尽管 MCU 表达和寡聚化完整，单向通道活性丢失。MCU 与 MICU1 和 MICU2 的交互需要 EMRE。因此，

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EMRE Is an Essential Component of the Mitochondrial Calcium Uniporter Complex

EMRE Emerges Concentrations of calcium within mitochondria are tightly regulated and modulate physiological mitochondrial functions, including control of metabolism and cell death. Sancak et al. (p. 1379, published online 14 November) complete the molecular characterization of the mitochondrial calcium uniporter (MCU), the multicomponent channel that allows concentration of calcium within the organelle. They identified a small protein termed “essential MCU regulator”—or EMRE—which was required for calcium transport activity of the fully assembled uniporter. A final but essential protein component involved in maintaining mitochondrial calcium levels is discovered. The mitochondrial uniporter is a highly selective calcium channel in the organelle’s inner membrane. Its molecular components include the EF-hand–containing calcium-binding proteins mitochondrial calcium uptake 1 (MICU1) and MICU2 and the pore-forming subunit mitochondrial calcium uniporter (MCU). We sought to achieve a full molecular characterization of the uniporter holocomplex (uniplex). Quantitative mass spectrometry of affinity-purified uniplex recovered MICU1 and MICU2, MCU and its paralog MCUb, and essential MCU regulator (EMRE), a previously uncharacterized protein. EMRE is a 10-kilodalton, metazoan-specific protein with a single transmembrane domain. In its absence, uniporter channel activity was lost despite intact MCU expression and oligomerization. EMRE was required for the interaction of MCU with MICU1 and MICU2. Hence, EMRE is essential for in vivo uniporter current and additionally bridges the calcium-sensing role of MICU1 and MICU2 with the calcium-conducting role of MCU.

更新日期：2013-11-14

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