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Boron-doped g-C3N4 supporting Cu nanozyme for colorimetric-fluorescent-smartphone detection of α-glucosidase
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2024-05-10 , DOI: 10.1016/j.aca.2024.342715 Qingjie Fu 1 , Shuang Liang 1 , Siqi Zhang 1 , Chenyu Zhou 1 , Yuntai Lv 1 , Xingguang Su 1
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2024-05-10 , DOI: 10.1016/j.aca.2024.342715 Qingjie Fu 1 , Shuang Liang 1 , Siqi Zhang 1 , Chenyu Zhou 1 , Yuntai Lv 1 , Xingguang Su 1
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Due to that the higher activity of nanozymes would bring outstanding performance for the nanozyme-based biosensing strategies, great efforts have been made by researchers to improve the catalytic activity of nanozymes, and novel nanozymes with high catalytic activity are desired. Considering the crucial role in controlling blood glucose level, strategies like colorimetric and chemiluminescence to monitor α-glucosidase are developed. However, multi-mode detection with higher sensitivity was insufficient. Therefore, developing triple-mode detection method for α-glucosidase based on great performance nanozyme is of great importance. In this work, a novel nanozyme Cu–BCN was synthesized by loading Cu on boron doped carbon substrate g-CN and applied to the colorimetric-fluorescent-smartphone triple-mode detection of α-glucosidase. In the presence of HO, Cu–BCN catalyzed the generation of O from HO, O subsequently oxidized TMB to blue colored oxTMB. In the presence of hydroquinone (HQ), the ROS produced from HO was consumed, inhibiting the oxidation of TMB, which endows the possibility of colorimetric and visual on-site detection of HQ. Further, due to that the fluorescence of Mg-CQDs at 444 nm could be quenched by oxTMB, HQ could also be quantified through fluorescent mode. Since α-glucosidase could efficiently hydrolyze α-arbutin into HQ, the sensitive detection of α-glucosidase was realized. Further, colorimetric paper-based device (-PAD) was fabricated for on-site α-glucosidase detection. The LODs for α-glucosidase via three modes were 2.20, 1.62 and 2.83 U/L respectively, high sensitivities were realized. The nanozyme Cu–BCN possesses higher peroxidase-like activity by doping boron to the substrate than non-doped Cu–CN. The proposed triple-mode detection of α-glucosidase is more sensitive than most previous reports, and is reliable when applied to practical sample. Further, the smartphone-based colorimetric paper-based analytical device (-PAD) made of simple materials could also detect α-glucosidase sensitively. The smartphone-based on-site detection provided a convenient, instrument-free and sensitive sensing method for α-glucosidase.
中文翻译：
硼掺杂 g-C3N4 支持铜纳米酶，用于比色荧光智能手机检测 α-葡萄糖苷酶
由于纳米酶的较高活性将为基于纳米酶的生物传感策略带来优异的性能，因此研究人员为提高纳米酶的催化活性付出了巨大的努力，并且需要具有高催化活性的新型纳米酶。考虑到控制血糖水平的关键作用，开发了比色法和化学发光等监测 α-葡萄糖苷酶的策略。然而，具有更高灵敏度的多模式检测还不够。因此，开发基于高性能纳米酶的α-葡萄糖苷酶三重模式检测方法具有重要意义。在这项工作中，通过将Cu负载在硼掺杂碳底物g-CN上合成了一种新型纳米酶Cu-BCN，并将其应用于α-葡萄糖苷酶的比色-荧光-智能手机三重模式检测。在 H2O 存在下，Cu-BCN 催化 H2O 生成 O，随后 O 将 TMB 氧化为蓝色 oxTMB。在对苯二酚（HQ）存在下，H2O产生的ROS被消耗，抑制了TMB的氧化，从而为HQ的比色和目视现场检测提供了可能。此外，由于Mg-CQDs在444 nm处的荧光可以被oxTMB猝灭，因此HQ也可以通过荧光模式进行定量。由于α-葡萄糖苷酶能够高效地将α-熊果苷水解为HQ，从而实现了α-葡萄糖苷酶的灵敏检测。此外，还制造了用于现场 α-葡萄糖苷酶检测的比色纸基装置（-PAD）。三种模式对α-葡萄糖苷酶的检测限分别为2.20、1.62和2.83 U/L，实现了高灵敏度。通过向底物掺杂硼，纳米酶 Cu-BCN 比未掺杂的 Cu-CN 具有更高的过氧化物酶样活性。 所提出的α-葡萄糖苷酶三重模式检测比大多数以前的报告更灵敏，并且在应用于实际样品时是可靠的。此外，由简单材料制成的基于智能手机的比色纸基分析装置（-PAD）也可以灵敏地检测α-葡萄糖苷酶。基于智能手机的现场检测为α-葡萄糖苷酶提供了一种便捷、免仪器且灵敏的传感方法。
更新日期：2024-05-10
中文翻译：
硼掺杂 g-C3N4 支持铜纳米酶，用于比色荧光智能手机检测 α-葡萄糖苷酶
由于纳米酶的较高活性将为基于纳米酶的生物传感策略带来优异的性能，因此研究人员为提高纳米酶的催化活性付出了巨大的努力，并且需要具有高催化活性的新型纳米酶。考虑到控制血糖水平的关键作用，开发了比色法和化学发光等监测 α-葡萄糖苷酶的策略。然而，具有更高灵敏度的多模式检测还不够。因此，开发基于高性能纳米酶的α-葡萄糖苷酶三重模式检测方法具有重要意义。在这项工作中，通过将Cu负载在硼掺杂碳底物g-CN上合成了一种新型纳米酶Cu-BCN，并将其应用于α-葡萄糖苷酶的比色-荧光-智能手机三重模式检测。在 H2O 存在下，Cu-BCN 催化 H2O 生成 O，随后 O 将 TMB 氧化为蓝色 oxTMB。在对苯二酚（HQ）存在下，H2O产生的ROS被消耗，抑制了TMB的氧化，从而为HQ的比色和目视现场检测提供了可能。此外，由于Mg-CQDs在444 nm处的荧光可以被oxTMB猝灭，因此HQ也可以通过荧光模式进行定量。由于α-葡萄糖苷酶能够高效地将α-熊果苷水解为HQ，从而实现了α-葡萄糖苷酶的灵敏检测。此外，还制造了用于现场 α-葡萄糖苷酶检测的比色纸基装置（-PAD）。三种模式对α-葡萄糖苷酶的检测限分别为2.20、1.62和2.83 U/L，实现了高灵敏度。通过向底物掺杂硼，纳米酶 Cu-BCN 比未掺杂的 Cu-CN 具有更高的过氧化物酶样活性。 所提出的α-葡萄糖苷酶三重模式检测比大多数以前的报告更灵敏，并且在应用于实际样品时是可靠的。此外，由简单材料制成的基于智能手机的比色纸基分析装置（-PAD）也可以灵敏地检测α-葡萄糖苷酶。基于智能手机的现场检测为α-葡萄糖苷酶提供了一种便捷、免仪器且灵敏的传感方法。
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