Genome analysis and description of Tunturibacter gen. nov. expands the diversity of Terriglobia in tundra soils,Environmental Microbiology

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Genome analysis and description of Tunturibacter gen. nov. expands the diversity of Terriglobia in tundra soils
Environmental Microbiology ( IF 4.3 ) Pub Date : 2024-05-22 , DOI: 10.1111/1462-2920.16640
Adriana Messyasz ₁ , Minna K Männistö ₂ , Lee J Kerkhof ₃ , Max M Häggblom ₁

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Increased temperatures in Arctic tundra ecosystems are leading to higher microbial respiration rates of soil organic matter, resulting in the release of carbon dioxide and methane. To understand the effects of this microbial activity, it is important to better characterize the diverse microbial communities in Arctic soil. Our goal is to refine our understanding of the phylogenetic diversity of Terriglobia, a common but elusive group within the Acidobacteriota phylum. This will help us link this diversity to variations in carbon and nitrogen usage patterns. We used long‐read Oxford Nanopore MinION sequences in combination with metagenomic short‐read sequences to assemble complete Acidobacteriota genomes. This allowed us to build multi‐locus phylogenies and annotate pangenome markers to distinguish Acidobacteriota strains from several tundra soil isolates. We identified a phylogenetic cluster containing four new species previously associated with Edaphobacter lichenicola. We conclude that this cluster represents a new genus, which we have named Tunturibacter. We describe four new species: Tunturibacter lichenicola comb. nov., Tunturibacter empetritectus sp. nov., Tunturibacter gelidoferens sp. nov., and Tunturibacter psychrotolerans sp. nov. By uncovering new species and strains within the Terriglobia and improving the accuracy of their phylogenetic placements, we hope to enhance our understanding of this complex phylum and shed light on the mechanisms that shape microbial communities in polar soils.

中文翻译：

Tunturibacter gen. 的基因组分析和描述。十一月扩大苔原土壤中陆生植物的多样性

北极苔原生态系统温度升高导致土壤有机质的微生物呼吸速率加快，导致二氧化碳和甲烷的释放。为了了解这种微生物活动的影响，更好地表征北极土壤中多样化的微生物群落非常重要。我们的目标是加深对系统发育多样性的理解土球属，一个常见但难以捉摸的群体酸杆菌门门。这将帮助我们将这种多样性与碳和氮使用模式的变化联系起来。我们使用长读长 Oxford Nanopore MinION 序列与宏基因组短读长序列相结合来组装完整的酸杆菌门基因组。这使我们能够构建多位点系统发育并注释泛基因组标记以区分酸杆菌门来自几种苔原土壤分离物的菌株。我们鉴定了一个包含四个新物种的系统发育簇，这些新物种以前与地衣埃达杆菌。我们得出结论，这个簇代表了一个新属，我们将其命名为钝土杆菌属。我们描述了四个新物种：地衣结核菌梳子。十一月，肠杆菌sp。十一月，凝胶管杆菌sp。十一月，和耐冷钝杆菌sp。十一月通过发现新的物种和菌株土球属并提高其系统发育位置的准确性，我们希望加深对这一复杂门的理解，并揭示塑造极地土壤微生物群落的机制。

更新日期：2024-05-22

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