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De novo transcriptome assembly of a lipoxygenase knock-down strain in the diatom Pseudo-nitzschia arenysensis
Scientific Data ( IF 5.8 ) Pub Date : 2024-05-22 , DOI: 10.1038/s41597-024-03375-0
Pina Marotta 1, 2 , Valeria Sabatino 1 , Luca Ambrosino 3 , Marco Miralto 3 , Maria Immacolata Ferrante 1, 4
Affiliation  

Diatoms are microalgae that live in marine and freshwater environments and are responsible for about 20% of the world’s carbon fixation. Population dynamics of these cells is finely regulated by intricate signal transduction systems, in which oxylipins are thought to play a relevant role. These are oxygenated fatty acids whose biosynthesis is initiated by a lipoxygenase enzyme (LOX) and are widely distributed in all phyla, including diatoms. Here, we present a de novo transcriptome obtained from the RNA-seq performed in the diatom species Pseudo-nitzschia arenysensis, using both a wild-type and a LOX-silenced strain, which will represent a reliable reference for comparative analyses within the Pseudo-nitzschia genus and at a broader taxonomic scale. Moreover, the RNA-seq data can be interrogated to go deeper into the oxylipins metabolic pathways.



中文翻译:


硅藻拟菱形藻中脂氧合酶敲低菌株的从头转录组组装



硅藻是生活在海洋和淡水环境中的微藻,其固碳量约占世界碳固定量的 20%。这些细胞的群体动态受到复杂的信号转导系统的精细调节,其中氧脂素被认为发挥了相关作用。这些是含氧脂肪酸,其生物合成由脂氧合酶 (LOX) 启动,广泛分布于包括硅藻在内的所有门中。在这里,我们展示了从硅藻物种Pseudo-nitzschia arenysensis中使用野生型和 LOX 沉默菌株进行的 RNA 测序获得的从头转录组,这将为伪菱形藻中的比较分析提供可靠的参考。菱形藻属和更广泛的分类学范围。此外,可以查询 RNA-seq 数据以更深入地了解氧脂质代谢途径。

更新日期:2024-05-22
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