AimTo examine association between subgingival microbial signatures and levels of cognitive impairment in older adults.Materials and MethodsWe analysed subgingival plaque samples and 16S ribosomal RNA sequences for microbiota among 165 participants (normal controls [NCs]: 40, subjective cognitive decline [SCD]: 40, mild cognitive impairment [MCI]: 49 and dementia: 36).ResultsThe bacterial richness was lower among individuals with worse cognitive function, and subgingival microbial communities differed significantly among the four groups. Declining cognitive function was associated with decreasing relative abundance of genera Capnocytophaga, Saccharibacteria_genera_incertae_sedis, Lautropia and Granulicatella, and increasing abundance of genus Porphyromonas. Moreover, there were differentially abundant genera among the groups. Random forest model based on subgingival microbiota could distinguish between cognitive impairment and NC (AUC = 0.933, 95% confidence interval 0.873–0.992). Significant correlations were observed between oral microbiota and sex, Montreal Cognitive Assessment (MoCA) score and Mini‐Mental State Examination score. Partial correlation analysis showed that Leptotrichia and Burkholderia were closely negatively associated with the MoCA score after adjusting for multiple covariates. Gene function was not significantly different between SCD and NC groups, whereas three homozygous genes were altered in MCI patients and two in dementia patients.ConclusionsThis is the first study to demonstrate an association between the composition, function and metabolic pathways of subgingival microbiota and different levels of cognitive function among older individuals. Future cohort studies should assess its diagnostic usefulness for cognitive impairment.

中文翻译：

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具有不同认知功能水平的老年人独特的龈下微生物特征


目的 研究老年人龈下微生物特征与认知障碍水平之间的关联。材料和方法我们分析了 165 名参与者的龈下菌斑样本和微生物群的 16S 核糖体 RNA 序列（正常对照 [NCs]：40，主观认知衰退 [SCD]：40） ，轻度认知障碍[MCI]：49和痴呆：36）。结果认知功能较差的个体中细菌丰富度较低，并且四组之间龈下微生物群落存在显着差异。认知功能下降与嗜二氧化碳菌属、糖杆菌属、不确定菌属、劳托皮亚属和颗粒菌属相对丰度的减少以及卟啉单胞菌属丰度的增加有关。此外，各类群之间存在丰富的属差异。基于龈下微生物群的随机森林模型可以区分认知障碍和NC（AUC = 0.933，95％置信区间0.873-0.992）。口腔微生物群与性别、蒙特利尔认知评估 (MoCA) 评分和简易精神状态检查评分之间存在显着相关性。偏相关分析表明，在调整多个协变量后，纤毛菌属和伯克霍尔德菌属与 MoCA 评分呈密切负相关。 SCD 组和 NC 组之间的基因功能没有显着差异，而 MCI 患者中有 3 个纯合基因发生改变，痴呆患者中有 2 个发生改变。结论这是第一项证明龈下微生物群的组成、功能和代谢途径与不同水平之间存在关联的研究老年人的认知功能。未来的队列研究应该评估其对认知障碍的诊断有用性。