Here, we present the concept of flat-field capillary-assisted nanoparticle tracking analysis for the characterization of fast diffusing nano-objects. By combining diffusion confinement and spatially invariant illumination, i.e., flat-fields, within a fiber-interfaced on-chip environment, ultra-long trajectories of fast diffusing objects within large microchannels have been measured via diffraction-limited imaging. Our study discusses the design procedure, explains potential limitations, and experimentally confirms flat-field formation by tracking gold nanospheres. The presented concept enables generating flat-fields in a novel on-chip optofluidic platform for the characterization of individual nano-objects for fundamental light/matter investigations or applications in bioanalytics and nanoscale material science.

中文翻译：

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毛细管辅助平场形成：在集成片上光流控环境中推进纳米颗粒跟踪分析的平台


在这里，我们提出了平场毛细管辅助纳米粒子跟踪分析的概念，用于表征快速扩散的纳米物体。通过在光纤接口片上环境中结合扩散限制和空间不变照明（即平场），可以通过衍射极限成像测量大型微通道内快速扩散物体的超长轨迹。我们的研究讨论了设计过程，解释了潜在的局限性，并通过跟踪金纳米球通过实验证实了平场的形成。所提出的概念能够在新型片上光流控平台中生成平场，用于表征单个纳米物体，用于基础光/物质研究或生物分析和纳米材料科学中的应用。