<br>SFGDI通过调节Sirt3/SOD/ROS通路和BV2细胞能量代谢对sirtuin 3相关氧化应激的神经保护作用,Food & Function

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SFGDI通过调节Sirt3/SOD/ROS通路和BV2细胞能量代谢对sirtuin 3相关氧化应激的神经保护作用
Food & Function ( IF 5.1 ) Pub Date : 2024-05-20 , DOI: 10.1039/d4fo01512f
Zhiqiang Lu _{1,

2} , Siqi Shen _{1,

2} , Songyi Lin _{1,

2}

Affiliation

最近，神经保护肽的研究在解决记忆障碍和认知能力下降方面引起了人们的关注。尽管已从海参中鉴定出潜在的神经保护肽丝氨酸-苯丙氨酸-甘氨酸-天冬氨酸-异亮氨酸（SFGDI），但其分子机制仍不清楚。本研究旨在探讨 SFGDI 对 BV2 细胞中 3-TYP 诱导的氧化应激的神经保护作用。结果显示，SFGDI在体外模拟胃肠消化过程中的保留率为76.70%，在大鼠外翻肠囊模型中的吸收率为10.41%。通过给小鼠灌胃给予 SFGDI 两小时后，在大脑中观察到显着的荧光，表明 SFGDI 通过与神经细胞的相互作用具有潜在的神经保护作用。通过利用 3-TYP 在 BV2 细胞中诱导的氧化应激损伤模型，确定用 SFGDI (50–200 μg mL ^-1 ) 预处理会导致乙酰化 SOD 水平呈剂量依赖性降低，从而导致 SOD 增强活性以及 ROS 和 MDA 水平降低。此外，这种预处理引发了不饱和脂质水平的增加，这有助于维持细胞内脂质代谢平衡，并保持线粒体功能和糖酵解水平以调节能量代谢。这项研究的结果表明，SFGDI 通过调节 Sirt3/SOD/ROS 通路、调节脂质代谢和增强 BV2 细胞的能量代谢而表现出神经保护特性。这些发现提出了解决 Sirt3 相关记忆缺陷和神经退行性疾病的潜在新治疗方法。

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The neuroprotective effects of SFGDI on sirtuin 3-related oxidative stress by regulating the Sirt3/SOD/ROS pathway and energy metabolism in BV2 cells

Recently, the investigation of neuroprotective peptides has gained attention in addressing memory impairment and cognitive decline. Although the potential neuroprotective peptide Serine–Phenylalanine–Glycine–Aspartic acid–Isoleucine (SFGDI) has been identified from sea cucumber, the molecular mechanisms remain unclear. This study was conducted to explore the neuroprotection of SFGDI against 3-TYP-induced oxidative stress in BV2 cells. The results showed a retention rate of 76.70% during in vitro simulated gastrointestinal digestion and an absorption rate of 10.41% in a rat-everted gut sac model for SFGDI. Two hours following the administration of SFGDI via gavage in mice, a notable fluorescence was observed in the brain, indicating a potential neuroprotection of SFGDI through its interactions with nerve cells. By utilizing a model of oxidative stress injury induced by 3-TYP in BV2 cells, it was determined that pretreatment with SFGDI (50–200 μg mL⁻¹) resulted in a dose-dependent reduction in the acetylated SOD level, leading to enhanced SOD activity and reduced levels of ROS and MDA. In addition, this pretreatment triggered an increase in unsaturated lipid levels, which helped maintain the intracellular lipid metabolism balance and preserve the mitochondrial function and glycolysis levels to regulate energy metabolism. The results of this study indicate that SFGDI demonstrates neuroprotective properties through its modulation of the Sirt3/SOD/ROS pathway, regulation of lipid metabolism, and enhancement of energy metabolism in BV2 cells. These findings suggest potential novel therapeutic approaches for addressing Sirt3-related memory deficits and neurodegenerative disorders.

更新日期：2024-05-20

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