The identification and detection of disease-related biomarkers is essential for early clinical diagnosis, evaluating disease progression, and for the development of therapeutics. Possessing the advantages of high sensitivity and selectivity, fluorescent probes have become effective tools for monitoring disease-related active molecules at the cellular level and in vivo. In this review, we describe current fluorescent probes designed for the detection and quantification of key bioactive molecules associated with common diseases, such as organ damage, inflammation, cancers, cardiovascular diseases, and brain disorders. We emphasize the strategies behind the design of fluorescent probes capable of disease biomarker detection and diagnosis and cover some aspects of combined diagnostic/therapeutic strategies based on regulating disease-related molecules. This review concludes with a discussion of the challenges and outlook for fluorescent probes, highlighting future avenues of research that should enable these probes to achieve accurate detection and identification of disease-related biomarkers for biomedical research and clinical applications.

中文翻译：

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用于疾病诊断的荧光探针


疾病相关生物标志物的识别和检测对于早期临床诊断、评估疾病进展和治疗方法的开发至关重要。荧光探针具有高灵敏度和选择性的优点，已成为在细胞水平和体内监测疾病相关活性分子的有效工具。在这篇综述中，我们描述了当前设计用于检测和定量与常见疾病相关的关键生物活性分子的荧光探针，例如器官损伤、炎症、癌症、心血管疾病和脑部疾病。我们强调能够进行疾病生物标志物检测和诊断的荧光探针设计背后的策略，并涵盖基于调节疾病相关分子的组合诊断/治疗策略的某些方面。本综述最后讨论了荧光探针的挑战和前景，强调了未来的研究途径，使这些探针能够准确检测和识别疾病相关的生物标志物，用于生物医学研究和临床应用。