To robustly discover and explore phytocompounds, it is necessary to evaluate the interrelationships between the plant species, plant tissue, and the extraction process on the extract composition and to predict its cytotoxicity. The present work evaluated how Fourier Transform InfraRed spectroscopy can acquire the molecular profile of aqueous and ethanol‐based extracts obtained from leaves, seeds, and flowers of Cynara Cardunculus, and ethanol‐based extracts from Matricaria chamomilla flowers, as well the impact of these extracts on the viability of mammalian cells. The extract molecular profile enabled to predict the extraction yield, and how the plant species, plant tissue, and extraction process affected the extract's relative composition. The molecular profile obtained from the culture media of cells exposed to extracts enabled to capture its impact on cells metabolism, at a higher sensitivity than the conventional assay used to determine the cell viability. Furthermore, it was possible to detect specific impacts on the cell's metabolism according to plant species, plant tissue, and extraction process. Since spectra were acquired on small volumes of samples (25 µL), after a simple dehydration step, and based on a plate with 96 wells, the method can be applied in a rapid, simple, high‐throughput, and economic mode, consequently promoting the discovery of phytocompounds.

中文翻译：

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一种快速、高通量比较植物提取物分子谱和生物活性的新方法


为了稳健地发现和探索植物化合物，有必要评估植物物种、植物组织和提取物成分的提取过程之间的相互关系，并预测其细胞毒性。目前的工作评估了傅里叶变换红外光谱如何获取从叶、种子和花中获得的水提取物和乙醇提取物的分子特征。菜蓟和乙醇提取物洋甘菊花，以及这些提取物对哺乳动物细胞活力的影响。提取物分子谱能够预测提取率，以及植物种类、植物组织和提取过程如何影响提取物的相对组成。从暴露于提取物的细胞培养基中获得的分子谱能够捕捉其对细胞代谢的影响，其灵敏度比用于确定细胞活力的传统测定更高。此外，还可以根据植物种类、植物组织和提取过程检测对细胞代谢的具体影响。由于光谱是在小体积样品（25 µL）上获得的，经过简单的脱水步骤，并且基于 96 孔板，该方法可以以快速、简单、高通量和经济的模式应用，从而促进植物化合物的发现。