<br>苦杏仁苷通过 JAK2/STAT3 通路抑制炎症改善 C3H/HeJ 小鼠斑秃,Journal of Ethnopharmacology

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苦杏仁苷通过 JAK2/STAT3 通路抑制炎症改善 C3H/HeJ 小鼠斑秃
Journal of Ethnopharmacology ( IF 4.8 ) Pub Date : 2024-05-08 , DOI: 10.1016/j.jep.2024.118317
Xun He ₁ , Jingsong Liu ₂ , Yugang Gong ₁ , Wei Lu ₁ , Xiaowei Sha ₁ , Chang Cao ₁ , Yanqun Li ₁ , Jiawei Wang ₃

Affiliation

有证据表明，中药方剂血府逐瘀汤能显着促进斑秃（AA）患者和小鼠新发的形成。苦杏仁苷是血府逐瘀汤的活性成分之一，但其对AA的治疗作用和潜在机制仍不清楚。因此，本研究旨在探讨其对C3H/HeJ小鼠AA模型的治疗效果，并探讨其炎症和免疫调节的分子机制。 C3H/HeJ雌性小鼠分为对照组、AA组、鲁索替尼组（60 mg/kg）和苦杏仁苷组（60、90和120 mg/kg，0.2 ml/10 g，ig）。用光学显微镜观察局部皮肤特征、胎毛、终毛的数量。进行H&E染色以确定皮肤病理损伤的程度。采用ELISA法检测小鼠血清中白细胞介素6（IL-6）、肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）的水平。流式细胞仪分析皮肤组织的CD4CD25FOXP3、CD4和CD8。免疫组化法检测CD4、CD8、p-JAK/JAK2、p-STAT3/STAT、SOCS3的水平。采用Western blot和qRT-PCR检测皮肤组织中IL-6、TNF-α、IFN-γ、JAK2、p-JAK、STAT、p-STAT3和SOCS3蛋白和基因的表达水平。通过皮肤显微镜和H&E染色测定，与AA组相比，苦杏仁苷极大地增加了毳毛的数量，并减少了终毛的数量。 ELISA、Western blot和qRT-PCR数据显示，AA小鼠血清和皮肤组织中IL-6、TNF-α和IFN-γ的水平显着升高，而苦杏仁苷显着抑制这三种促炎物质的含量。因素。流式细胞术和免疫组织化学结果表明，苦杏仁苷可显着增加 AA 小鼠 CD4CD25FOXP3 和 CD4 细胞的数量，同时抑制 CD8 阳性细胞的数量。此外，苦杏仁苷显着降低 AA 小鼠中 JAK2/STAT3 通路相关蛋白和基因水平。苦杏仁苷在AA治疗中可以抑制炎症反应、改善免疫功能。其分子机制可能与抑制JAK2/STAT3通路有关。

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Amygdalin ameliorates alopecia areata on C3H/HeJ mice by inhibiting inflammation through JAK2/STAT3 pathway

Evidence has demonstrated that Chinese medicine formula Xuefu Zhuyu decoction can markedly promote the formation of new hair in patients and mice with alopecia areata (AA). Amygdalin is one of the active components of Xuefu Zhuyu decoction, but its therapeutic effects and the underlying mechanisms on AA remains largely unrevealed. Therefore, this study aims to investigate the therapeutic effects and to probe its molecular mechanisms of inflammation and immune regulation on AA model of C3H/HeJ mice. The C3H/HeJ female mice were divided into control, AA, rusolitinib (60 mg/kg), and amygdalin groups (60, 90, and 120 mg/kg, 0.2 ml/10 g, i.g.). The optical microscope was used to observe the feature of the local skin, and the number of lanugo and terminal hair. H&E staining was performed to determine the degree of pathological damage to the skin. ELISA was performed to detect levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in mice serum. Flow cytometry was carried out to analyze the CD4CD25FOXP3, CD4 and CD8 of skin tissue. And the levels of CD4 and CD8, p-JAK/JAK2, p-STAT3/STAT, and SOCS3 were detected by immunohistochemistry. Western blot and qRT-PCR were employed to examine the expression levels of IL-6, TNF-α, IFN-γ, JAK2, p-JAK, STAT, p-STAT3 and SOCS3 proteins and genes in skin tissues. Compared with AA group, amygdalin immensely increased the number of vellus hairs and decreased the number of terminal hairs determined by skin microscopy and H&E staining. ELISA, Western blot and qRT-PCR data showed that the levels of IL-6, TNF-α and IFN-γ in serum and skin tissues of AA mice were significantly increased, while amygdalin administration dramatically restrained the contents of the three pro-inflammatory factors. Flow cytometry and immunohistochemistry hinted that amygdalin observably enhanced the number of CD4CD25FOXP3 and CD4 cells, while inhibited the number of CD8 positive cells in mice with AA. Moreover, amygdalin signally reduced JAK2/STAT3 pathway-related protein and gene levels in AA mice. Amygdalin could inhibit inflammatory response and improve immune function in the treatment of AA. The underlying molecular mechanism may be related to inhibition of JAK2/STAT3 pathway.

更新日期：2024-05-08

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