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Nanobodies-based colloidal gold immunochromatographic assay for specific detection of parathion
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2024-05-10 , DOI: 10.1016/j.aca.2024.342717 Mingwei Guo 1 , Jiarui Pang 1 , Yu Wang 2 , Chaohui Bi 1 , Zhenlin Xu 1 , Yudong Shen 1 , Jinyi Yang 1 , Hong Wang 1 , Yuanming Sun 1
Analytica Chimica Acta ( IF 5.7 ) Pub Date : 2024-05-10 , DOI: 10.1016/j.aca.2024.342717 Mingwei Guo 1 , Jiarui Pang 1 , Yu Wang 2 , Chaohui Bi 1 , Zhenlin Xu 1 , Yudong Shen 1 , Jinyi Yang 1 , Hong Wang 1 , Yuanming Sun 1
Affiliation
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Parathion is one of organophosphorus pesticide, which has been prohibited in agricultural products due to its high toxicity to human beings. However, there are still abuse cases for profit in agricultural production. Hence, we established nanobodies-based colloidal gold immunochromatographic assay (GICA) in which nanobodies (Nbs) as an excellent recognition element, greatly improving the stability and sensitivity of ICA. Under the optimal conditions, the developed Nbs-based GICA showed a cut-off value of 50 ng/mL for visual judgment and a half-inhibitory concentration (IC) of 2.39 ng/mL for quantitative detection. The limit of detection (LOD) was as low as 0.15 ng/mL which was significantly 50-fold higher sensitivity than the commercial mAb-ICA. Additionally, this method exhibited good recoveries for the detection of cabbage, cucumber, and orange samples and excellent correlation with the UPLC-MS/MS method. The results showed that this method developed in this work based on nanobody can be used in practical detection of parathion in foods and nanobody is novel prospective antibody resource for immunoassays of chemical contaminants.
中文翻译：
基于纳米抗体的胶体金免疫层析法特异性检测对硫磷
对硫磷是有机磷农药之一，由于对人体毒性较大，已被禁止在农产品中使用。但在农业生产中，牟取暴利的现象仍然存在。因此，我们建立了基于纳米抗体的胶体金免疫色谱法（GICA），其中纳米抗体（Nbs）作为优异的识别元件，大大提高了ICA的稳定性和灵敏度。在最佳条件下，开发的基于Nbs的GICA显示出用于目视判断的50 ng/mL的截止值和用于定量检测的2.39 ng/mL的半抑制浓度（IC）。检测限 (LOD) 低至 0.15 ng/mL，灵敏度比商用 mAb-ICA 高出 50 倍。此外，该方法在检测卷心菜、黄瓜和橙子样品时表现出良好的回收率，并且与 UPLC-MS/MS 方法具有良好的相关性。结果表明，本工作开发的基于纳米抗体的方法可用于食品中对硫磷的实际检测，纳米抗体是化学污染物免疫分析的新型前瞻性抗体资源。
更新日期：2024-05-10
中文翻译：
基于纳米抗体的胶体金免疫层析法特异性检测对硫磷
对硫磷是有机磷农药之一，由于对人体毒性较大，已被禁止在农产品中使用。但在农业生产中，牟取暴利的现象仍然存在。因此，我们建立了基于纳米抗体的胶体金免疫色谱法（GICA），其中纳米抗体（Nbs）作为优异的识别元件，大大提高了ICA的稳定性和灵敏度。在最佳条件下，开发的基于Nbs的GICA显示出用于目视判断的50 ng/mL的截止值和用于定量检测的2.39 ng/mL的半抑制浓度（IC）。检测限 (LOD) 低至 0.15 ng/mL，灵敏度比商用 mAb-ICA 高出 50 倍。此外，该方法在检测卷心菜、黄瓜和橙子样品时表现出良好的回收率，并且与 UPLC-MS/MS 方法具有良好的相关性。结果表明，本工作开发的基于纳米抗体的方法可用于食品中对硫磷的实际检测，纳米抗体是化学污染物免疫分析的新型前瞻性抗体资源。
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