Because of the growing high importance of the development of biocatalytic fuel cell (FC) technologies for renewable energy-producing and testing systems for medical or environmental purposes, in this study, we constructed and demonstrated an H FC voltammeter working with graphite sample testing micro-strips and based on microbial cells. Presented H FC voltammeter that provides fast and precise testing of bio-electrochemical possible reactions in biosamples for H and other gases, is automated with software which works in NI LabVIEW programming environment, has amplifier cascade system with high internal resistance, temperature controlling and resistance cascade. Microbial Hydrogenase (Hyd) enzymes reversibly catalyze the formation and oxidation of H. Isolation and characterization of O-tolerant [NiFe]-hydrogenases (Hyds) have given rise to new concepts in H FC. and [NiFe]-Hyds can be applied as a biocatalyst anode in biofuel cells (BFCs). We evaluated the efficiency of applying the 3 µl (1.5 mg cell dry weight) intact cells or crude extracts on 0.5 cm as anode catalyzers in the bio-electrochemical system. The highest electrical potential (up to 0.7 V) was achieved with bacterial whole cells, which were grown on glucose and glycerol.

中文翻译：

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开发由大肠杆菌细胞驱动的 H2 燃料电池电化学系统

由于生物催化燃料电池 (FC) 技术的开发对于医疗或环境用途的可再生能源生产和测试系统的重要性日益提高，在这项研究中，我们构建并演示了一种与石墨样品测试微量配合使用的 H FC 伏安计。条带并基于微生物细胞。推出的 H FC 伏安计可快速、精确地测试生物样品中 H 和其他气体的生物电化学可能反应，通过在 NI LabVIEW 编程环境中运行的软件实现自动化，具有具有高内阻、温度控制和电阻级联的放大器级联系统。微生物氢化酶 (Hyd) 可逆地催化 H 的形成和氧化。耐氧 [NiFe]-氢化酶 (Hyds) 的分离和表征引发了 HFC 的新概念。 [NiFe]-Hyds 可用作生物燃料电池（BFC）中的生物催化剂阳极。我们评估了在 0.5 cm 上应用 3 µl（1.5 mg 细胞干重）完整细胞或粗提物作为生物电化学系统中阳极催化剂的效率。在葡萄糖和甘油上生长的细菌全细胞达到了最高电势（高达 0.7 V）。