<br>电纺壳聚糖/聚乙烯醇/蒙脱石粘土支架与 l929 细胞系在皮肤再生活性和硅分子对接研究中的细胞相容性和细胞增殖评价,International Journal of Biological Macromolecules

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电纺壳聚糖/聚乙烯醇/蒙脱石粘土支架与 l929 细胞系在皮肤再生活性和硅分子对接研究中的细胞相容性和细胞增殖评价
International Journal of Biological Macromolecules ( IF 7.7 ) Pub Date : 2024-04-22 , DOI: 10.1016/j.ijbiomac.2024.131762
K Sangeetha ₁ , Mohammed F Albeshr ₂ , K Shoba ₃ , G Lavanya ₄ , P Supriya Prasad ₅ , P N Sudha ₆

Affiliation

本研究描述了采用静电纺丝方法开发新型壳聚糖/聚乙烯醇/蒙脱石粘土（CS/PVA/MMT）支架，并用L929成纤维细胞系评估其生物相容性，以确定其在伤口愈合应用中的用途。使用分析技术对制造的支架进行表征。 FT-IR 测量显示相关官能团的存在，并且 XRD 表明支架的无定形性质。使用 TEM 和 SEM 评估支架的形态和孔径。所制备的支架的孔径约为125 nm。针对选定的病原体对支架的抗菌测定进行了评估，结果表明具有更高的抗菌功效。使用DPPH测定测试的清除活性显示出显着的清除能力。通过 L929 试验进行的细胞毒性试验测试了伤口愈合特性，证明支架是适合细胞增殖的材料。此外，使用人中性粒细胞弹性蛋白酶（HNE）1H1B蛋白作为CB-Dock服务器中的受体，对CS/PVA/MMT配体进行了分子对接研究。进行的研究表明，CS/PVA/MMT 配体与人中性粒细胞弹性蛋白酶 (HNE) 1H1B 蛋白的关键残基存在强相互作用和高结合能，有助于组织再生活性。

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Evaluation of cytocompatibility and cell proliferation of electrospun chitosan/polyvinyl alcohol/montmorillonite clay scaffold with l929 cell lines in skin regeneration activity and in silico molecular docking studies

The present investigation describes the development of a novel Chitosan/Polyvinyl Alcohol/Montmorillonite Clay (CS/PVA/MMT) scaffold by adopting an electrospinning method, and their biocompatibility was evaluated with L929 fibroblast cell line to ascertain its use in wound healing applications. The fabricated scaffold was characterized using analytical techniques. FT-IR measurement exhibited the existence of relevant functional groups and XRD implies scaffolds' amorphous nature. The scaffold's morphology and pore diameter were assessed using TEM and SEM. The pore diameter of the as-prepared scaffold was approximately 125 nm. The antimicrobial assay of the scaffold was evaluated against selected pathogens which demonstrated higher antimicrobial efficacy. The scavenging activity tested using the DPPH assay showed remarkable scavenging capability. The wound healing properties were tested through the Cytotoxicity assay conducted on the L929 assay which proved the scaffold to be a suitable material for cell proliferation. Also, a Molecular docking investigation was carried out for CS/PVA/MMT ligand using human neutrophil elastase (HNE) 1H1B protein as a receptor in the CB-Dock server. Studies conducted revealed strong interaction and high binding energy ratings of CS/PVA/MMT ligand with key residues of human neutrophil elastase (HNE) 1H1B proteins that help in tissue regeneration activity.

更新日期：2024-04-22

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