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Versatile Platinum Nanoparticles-Decorated Phage Nanozyme Integrating Recognition, Bacteriolysis, and Catalysis Capabilities for On-Site Detection of Foodborne Pathogenic Strains Vitality Based on Bioluminescence/Pressure Dual-Mode Bioassay
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-05-10 , DOI: 10.1021/acs.analchem.4c01192 Hang You 1 , Nannan Ma 1 , Tianhua Li 1 , Zhenzhong Yu 1 , Ning Gan 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-05-10 , DOI: 10.1021/acs.analchem.4c01192 Hang You 1 , Nannan Ma 1 , Tianhua Li 1 , Zhenzhong Yu 1 , Ning Gan 1
Affiliation
Sensitive and on-site discrimination of live and dead foodborne pathogenic strains remains a significant challenge due to the lack of appropriate assay and signal probes. In this work, a versatile platinum nanoparticle-decorated phage nanozyme (P2@PtNPs) that integrated recognition, bacteriolysis, and catalysis was designed to establish the bioluminescence/pressure dual-mode bioassay for on-site determination of the vitality of foodborne pathogenic strains. Benefiting from the bacterial strain-level specificity of phage, the target Salmonella typhimurium (S.T) was specially captured to form sandwich complexes with P2@PtNPs on another phage-modified glass microbead (GM@P1). As the other part of the P2@PtNPs nanozyme, the introduced PtNPs could not only catalyze the decomposition of hydrogen peroxide to generate a significant oxygen pressure signal but also produce hydroxyl radicals around the target bacteria to enhance the bacteriolysis of phage and adenosine triphosphate release. It significantly improved the bioluminescence signal. The two signals corresponded to the total and live target bacteria counts, so the dead target could be easily calculated from the difference between the total and live target bacteria counts. Meanwhile, the vitality of S.T was realized according to the ratio of live and total S.T. Under optimal conditions, the application range of this proposed bioassay for bacterial vitality was 102–107 CFU/mL, with a limit of detections for total and live S.T of 30 CFU/mL and 40 CFU/mL, respectively. This work provides an innovative and versatile nanozyme signal probe for the on-site determination of bacterial vitality for food safety.
中文翻译:
多功能铂纳米颗粒修饰的噬菌体纳米酶集成识别、溶菌和催化功能,基于生物发光/压力双模式生物测定法现场检测食源性致病菌株活力
由于缺乏适当的检测和信号探针,对活的和死的食源性致病菌株的灵敏和现场区分仍然是一个重大挑战。在这项工作中,设计了一种集识别、溶菌和催化于一体的多功能铂纳米颗粒修饰的噬菌体纳米酶(P2@PtNPs),旨在建立生物发光/压力双模式生物测定法,用于现场测定食源性致病菌株的活力。受益于噬菌体的菌株水平特异性,目标鼠伤寒沙门氏菌(ST)被专门捕获,与另一个噬菌体修饰的玻璃微珠(GM@P1)上的P2@PtNPs形成夹心复合物。作为P2@PtNPs纳米酶的另一部分,引入的PtNPs不仅可以催化过氧化氢分解产生显着的氧压信号,而且可以在目标细菌周围产生羟基自由基,增强噬菌体的溶菌和三磷酸腺苷的释放。它显着改善了生物发光信号。这两个信号对应于目标细菌总数和活目标细菌计数,因此可以根据目标细菌总数和活目标细菌计数之间的差异轻松计算出死亡目标。同时,ST的活力是根据活ST和总ST的比例来实现的。在最佳条件下,该细菌活力生物测定的应用范围为10 2 –10 7 CFU/mL,总和活ST的检测限为10 2 –10 7 CFU/mL。 ST 分别为 30 CFU/mL 和 40 CFU/mL。这项工作提供了一种创新且多功能的纳米酶信号探针,用于现场测定细菌活力以确保食品安全。
更新日期:2024-05-10
中文翻译:
多功能铂纳米颗粒修饰的噬菌体纳米酶集成识别、溶菌和催化功能,基于生物发光/压力双模式生物测定法现场检测食源性致病菌株活力
由于缺乏适当的检测和信号探针,对活的和死的食源性致病菌株的灵敏和现场区分仍然是一个重大挑战。在这项工作中,设计了一种集识别、溶菌和催化于一体的多功能铂纳米颗粒修饰的噬菌体纳米酶(P2@PtNPs),旨在建立生物发光/压力双模式生物测定法,用于现场测定食源性致病菌株的活力。受益于噬菌体的菌株水平特异性,目标鼠伤寒沙门氏菌(ST)被专门捕获,与另一个噬菌体修饰的玻璃微珠(GM@P1)上的P2@PtNPs形成夹心复合物。作为P2@PtNPs纳米酶的另一部分,引入的PtNPs不仅可以催化过氧化氢分解产生显着的氧压信号,而且可以在目标细菌周围产生羟基自由基,增强噬菌体的溶菌和三磷酸腺苷的释放。它显着改善了生物发光信号。这两个信号对应于目标细菌总数和活目标细菌计数,因此可以根据目标细菌总数和活目标细菌计数之间的差异轻松计算出死亡目标。同时,ST的活力是根据活ST和总ST的比例来实现的。在最佳条件下,该细菌活力生物测定的应用范围为10 2 –10 7 CFU/mL,总和活ST的检测限为10 2 –10 7 CFU/mL。 ST 分别为 30 CFU/mL 和 40 CFU/mL。这项工作提供了一种创新且多功能的纳米酶信号探针,用于现场测定细菌活力以确保食品安全。