GCN5L1, also known as BLOC1S1 and BLOS1, is a small intracellular protein involved in many key biological processes. Over the last decade, GCN5L1 has been implicated in the regulation of protein lysine acetylation, energy metabolism, endo-lysosomal function, and cellular immune pathways. An increasing number of published papers have used commercially-available reagents to interrogate GCN5L1 function. However, in many cases these reagents have not been rigorously validated, leading to potentially misleading results. In this report we tested several commercially-available antibodies for GCN5L1, and found that two-thirds of those available did not unambiguously detect the protein by western blot in cultured mouse cells or ex vivo liver tissue. These data suggest that previously published studies which used these unverified antibodies to measure GCN5L1 protein abundance, in the absence of other independent methods of corroboration, should be interpreted with appropriate caution.

中文翻译：

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使用敲除细胞和组织验证 GCN5L1/BLOC1S1/BLOS1 抗体


GCN5L1，也称为 BLOC1S1 和 BLOS1，是一种参与许多关键生物过程的细胞内小蛋白。在过去的十年中，GCN5L1 一直与蛋白质赖氨酸乙酰化、能量代谢、内溶酶体功能和细胞免疫途径的调节有关。越来越多的已发表论文使用市售试剂来研究 GCN5L1 功能。然而，在许多情况下，这些试剂没有经过严格验证，可能导致误导性结果。在本报告中，我们测试了几种市售的 GCN5L1 抗体，发现三分之二的可用抗体并未通过培养的小鼠细胞或离体肝组织中的蛋白质印迹明确检测到该蛋白质。这些数据表明，在没有其他独立证实方法的情况下，先前发表的使用这些未经验证的抗体测量 GCN5L1 蛋白丰度的研究应适当谨慎地解释。