Non-smokers exposed to second-hand smoke (SHS) present risk of developing tobacco smoke-associated pathologies. To investigate the airway molecular response to SHS exposure that could be used in health risk assessment, comparative shotgun proteomics was performed on nasal epithelium from a group of healthy restaurant workers, non-smokers (never and former) exposed and not exposed to SHS in the workplace. HIF1α-glycolytic targets (GAPDH, TPI) and proteins related to xenobiotic metabolism, cell proliferation and differentiation leading to cancer (ADH1C, TUBB4B, EEF2) showed significant modulation in non-smokers exposed. In never smokers exposed, enrichment of glutathione metabolism pathway and EEF2-regulating protein synthesis in genotoxic response were increased, while in former smokers exposed, proteins (LYZ, ATP1A1, SERPINB3) associated with tissue damage/regeneration, apoptosis inhibition and inflammation that may lead to asthma, COPD or cancer, were upregulated. The identified proteins are potential response and susceptibility/risk biomarkers for SHS exposure.

中文翻译：

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职业二手烟暴露：对健康餐厅工作人员鼻上皮的比较鸟枪蛋白质组学研究


接触二手烟 (SHS) 的非吸烟者存在患烟草烟雾相关病症的风险。为了研究可用于健康风险评估的对二手烟暴露的气道分子反应，对一组健康餐厅工作人员、不吸烟者（从未和以前）暴露于和未暴露于二手烟的鼻上皮进行了鸟枪法蛋白质组学比较。工作场所。 HIF1α-糖酵解靶点（GAPDH、TPI）和与异生物质代谢、细胞增殖和分化相关的蛋白质（ADH1C、TUBB4B、EEF2）在暴露的非吸烟者中表现出显着的调节。在从未吸烟者中，谷胱甘肽代谢途径的富集和基因毒性反应中 EEF2 调节蛋白合成增加，而在曾经吸烟者中，与组织损伤/再生、细胞凋亡抑制和炎症相关的蛋白质（LYZ、ATP1A1、SERPINB3）增加，可能导致组织损伤/再生、细胞凋亡抑制和炎症。哮喘、慢性阻塞性肺病或癌症的风险均上调。已鉴定的蛋白质是二手烟暴露的潜在反应和易感性/风险生物标志物。