Acinetobacter baumannii biofilm was inhibited by tryptanthrin through disrupting its different stages and genes expression,iScience

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Acinetobacter baumannii biofilm was inhibited by tryptanthrin through disrupting its different stages and genes expression
iScience ( IF 4.6 ) Pub Date : 2024-05-08 , DOI: 10.1016/j.isci.2024.109942
Tingting Guo _{1,

2,

3} , Na Zhou ₁ , Liying Yang ₁ , Zichen Wang ₁ , Changchao Huan ₄ , Tao Lin ₅ , Guangyu Bao ₅ , Jian Hu ₆ , Guocai Li _{1,

2,

3}

Affiliation

Biofilm formation plays a significant role in antibiotic resistance, necessitating the search for alternative therapies against biofilm-associated infections. This study demonstrates that 20 μg/mL tryptanthrin can hinder biofilm formation above 50% in various A. baumannii strains. Tryptanthrin impacts various stages of biofilm formation, including the inhibition of surface motility and eDNA release in A. baumannii, as well as an increase in its sensitivity to H202. RT-qPCR analysis reveals that tryptanthrin significantly decreases the expression of the following genes: abaI (19.07%), abaR (33.47%), bfmR (43.41%), csuA/B (64.16%), csuE (50.20%), ompA (67.93%), and katE (72.53%), which are related to biofilm formation and quorum sensing. Furthermore, tryptanthrin is relatively safe and can reduce the virulence of A. baumannii in a Galleria mellonella infection model. Overall, our study demonstrates the potential of tryptanthrin in controlling biofilm formation and virulence of A. baumannii by disrupting different stages of biofilm formation and intercellular signaling communication.

中文翻译：

鲍曼不动杆菌生物膜通过破坏色当菊酯的不同阶段和基因表达而被色当菊酯抑制

生物膜的形成在抗生素耐药性中起着重要作用，因此需要寻找针对生物膜相关感染的替代疗法。本研究表明，20 μg/mL 色钽菊酯可阻止各种鲍曼不动杆菌菌株中 50% 以上的生物膜形成。色钽菊酯影响生物膜形成的各个阶段，包括抑制鲍曼不动杆菌的表面运动和 eDNA 释放，以及增加其对 H202 的敏感性。RT-qPCR 分析显示，色坦菊酯显著降低以下基因的表达：abaI （19.07%）、abaR （33.47%）、bfmR （43.41%）、csuA/B （64.16%）、csuE （50.20%）、ompA （67.93%）和 katE （72.53%），这些基因与生物膜形成和群体感应有关。此外，色钽菊酯相对安全，可以在 Galleria mellonella 感染模型中降低鲍曼不动杆菌的毒力。总体而言，我们的研究证明了色钽菊酯通过破坏生物膜形成和细胞间信号传导的不同阶段来控制鲍曼不动杆菌生物膜形成和毒力的潜力。

更新日期：2024-05-08

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