In this communication, local electrochemiluminescence (ECL) imaging is achieved using scanning electrochemical cell microscopy (SECCM) to create a localized region for the occurrence of ECL reaction. The system is demonstrated using a droplet at the micropipette that is contact with the prepared Ru-Silica@Au particles at indium tin oxide (ITO) slide. Tri--propylamine (TPrA) in the droplet reacts with ruthenium complex in a 10 μm-diameter-region to produce ECL emission, which is recorded by photo multiplier tube (PMT) underneath the ITO slide. By contacting each local region consecutively, the distribution of steady-state current and the related ECL emission at the ITO surface is imaged. The local ECL intensity show an approximately linear dependence with the current, but obvious deviation from the linear curve is observed at some regions. This result suggests that the ECL emission is not only determined by the transferred electron number in the electrochemical reaction, and also affected by local environments at ITO surface. As compared with the traditionally used ECL imaging in the bulk solution, this strategy avoids the cross-talking of ECL emission from nearby regions, and could offer a new way to unveil more molecular mechanism in the ECL reaction.

中文翻译：

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使用扫描电化学细胞显微镜进行局部电化学发光成像


在此通讯中，使用扫描电化学细胞显微镜 (SECCM) 实现局部电化学发光 (ECL) 成像，以创建发生 ECL 反应的局部区域。该系统通过微量移液器上的液滴与氧化铟锡 (ITO) 载玻片上制备的 Ru-Silica@Au 颗粒接触进行演示。液滴中的三丙胺 (TPrA) 与直径 10 μm 区域内的钌络合物反应，产生 ECL 发射，并由 ITO 载玻片下方的光电倍增管 (PMT) 记录。通过连续接触每个局部区域，对 ITO 表面的稳态电流分布和相关 ECL 发射进行成像。局部ECL强度与电流呈近似线性相关，但在某些区域观察到与线性曲线的明显偏差。这一结果表明，ECL发射不仅取决于电化学反应中转移的电子数，还受到ITO表面局部环境的影响。与传统在本体溶液中使用的ECL成像相比，该策略避免了附近区域的ECL发射的串扰，并且可以提供一种新的方法来揭示ECL反应中的更多分子机制。