To investigate the global transcriptional landscape of lacrimal gland cell populations in the GVHD mouse model. Single-cell RNA sequencing and further bioinformatic analysis of dissociated lacrimal gland (LG) cells from the mouse model were performed. Parts of transcriptional results were confirmed by immunofluorescence staining. We identified 23 cell populations belonging to 11 cell types. In GVHD LG, the proportion of acinar cells, myoepithelial cells, and endothelial cells was remarkably decreased, while T cells and macrophages were significantly expanded. Gene expression analysis indicated decreased secretion function, extracellular matrix (ECM) synthesis, and increased chemokines of myoepithelial cells. A newly described epithelial population named epithelial cells, expressing distinct gene signatures, was exclusively identified in GVHD LG. The fibroblasts exhibited an inflammation gene pattern. The gene pattern of endothelial cells suggested an increased ability to recruit immune cells and damaged cell-cell junctions. T cells were mainly comprised of Th2 cells and effective memory CD8 T cells. GVHD macrophages exhibited a Th2 cell-linked pattern. This single-cell atlas uncovered alterations of proportion and gene expression patterns of cell populations and constructed cell-cell communication networks of GVHD LG. These data may provide some new insight into understanding the development of ocular GVHD.

中文翻译：

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单细胞 RNA 测序揭示 GVHD 小鼠模型泪腺的转录景观


研究 GVHD 小鼠模型中泪腺细胞群的整体转录状况。对小鼠模型中分离的泪腺 (LG) 细胞进行了单细胞 RNA 测序和进一步的生物信息学分析。部分转录结果通过免疫荧光染色得到证实。我们鉴定了属于 11 种细胞类型的 23 个细胞群。在GVHD LG中，腺泡细胞、肌上皮细胞和内皮细胞的比例显着减少，而T细胞和巨噬细胞显着扩增。基因表达分析表明肌上皮细胞的分泌功能、细胞外基质（ECM）合成下降和趋化因子增加。在 GVHD LG 中专门鉴定出一种新描述的上皮细胞群，称为上皮细胞，表达不同的基因特征。成纤维细胞表现出炎症基因模式。内皮细胞的基因模式表明募集免疫细胞和受损细胞-细胞连接的能力增强。 T细胞主要由Th2细胞和有效记忆CD8 T细胞组成。 GVHD 巨噬细胞表现出 Th2 细胞连锁模式。该单细胞图谱揭示了细胞群比例和基因表达模式的变化，并构建了 GVHD LG 的细胞间通讯网络。这些数据可能为了解眼部 GVHD 的发展提供一些新的见解。