<br>DEHTP 对斑马鱼 (Danio rerio) 不同生命阶段甲状腺和性激素的干扰作用,Chemosphere

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DEHTP 对斑马鱼 (Danio rerio) 不同生命阶段甲状腺和性激素的干扰作用
Chemosphere ( IF 8.1 ) Pub Date : 2024-04-22 , DOI: 10.1016/j.chemosphere.2024.142105
Yunchul Ihn ₁ , Yoojin Cho ₁ , Yura Lee ₁ , Hyesun Seok ₁ , Jin-Su Oh ₂ , Hyo-Bang Moon ₂ , Kyungho Choi ₃

Affiliation

对苯二甲酸二(2-乙基己基)酯 (DEHTP) 是一种广泛用于众多消费品的替代增塑剂，取代邻苯二甲酸二(2-乙基己基)酯 (DEHP)。因此，DEHTP 在环境和人类中经常被检测到。作为 DEHP 的结构异构体和功能类似物，DEHTP 是一种疑似内分泌干扰物。在这里，我们使用胚胎幼虫和成年雄性斑马鱼评估了 DEHTP 的甲状腺干扰作用。我们还研究了它对成年斑马鱼性激素的破坏潜力。暴露于 DEHTP 5 天和 7 天后，幼鱼体内的甲状腺激素水平显着增加。观察到一些甲状腺调节基因的下调，包括、、和，但仅在暴露 5 天后才观察到。经过 21 天的暴露后，成年雄性斑马鱼的总三碘甲状腺原氨酸显着下降，而促甲状腺激素则增加。在两种脱碘酶和的上调以及的下调的支持下，甲状腺激素脱碘的潜在变化可以解释成年斑马鱼的甲状腺激素变化。此外，在成年斑马鱼中观察到雌二醇和 11-酮睾酮显着下降，同时睾酮 (T) 增加。几个类固醇生成基因的上调可以解释 T 升高，而确切的作用机制值得进一步研究。我们的结果表明，DEHTP 会导致斑马鱼不同生命阶段的甲状腺和性激素紊乱。

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Thyroid and sex hormone disrupting effects of DEHTP at different life stages of zebrafish (Danio rerio)

Di(2-ethylhexyl) terephthalate (DEHTP) is an alternative plasticizer widely used in numerous consumer products, replacing di(2-ethylhexyl) phthalate (DEHP). Hence, DEHTP has been frequently detected in the environment and humans. As a structural isomer and functional analog of DEHP, DEHTP is a suspected endocrine disruptor. Here, we evaluated thyroid-disrupting effects of DEHTP using embryo-larval and adult male zebrafish. We also investigated its sex hormone disruption potential in the adult zebrafish. After 5- and 7-days of exposure to DEHTP, significant increases in whole-body thyroid hormonal levels were observed in the larval fish. Down-regulation of several thyroid-regulating genes, including , , , and , was observed, but only after 5-day exposure. Following a 21-day exposure, the adult male zebrafish exhibited a significant decrease in total triiodothyronine and an increase in thyroid-stimulating hormones. Potential changes in the deiodination of thyroid hormones, supported by the up-regulation of two deiodinases, and , along with the down-regulation of , could explain the thyroid hormone changes in the adult zebrafish. Moreover, significant trends of decrease in estradiol and 11-ketotestosterone, along with increase of testosterone (T), were observed in the adult zebrafish. Up-regulation of several steroidogenic genes may explain elevated T, while exact mechanisms of action warrant further investigation. Our results demonstrate that DEHTP can cause disruptions of thyroid and sex hormones at different life stages in zebrafish.

更新日期：2024-04-22

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