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A Kumujian-C based highly selective fluorescence turn-on probe enables the detection of sulfite in real samples and living cells
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2024-05-03 , DOI: 10.1039/d4nj01136h Kartik Dutta 1 , Saparya Chattaraj 2, 3 , Riddhi Pal 1 , Padma Nilaya Jonnalgadda 2, 4 , Birija S. Patro 1, 2 , Soumyaditya Mula 1, 2 , Goutam Chakraborty 4
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2024-05-03 , DOI: 10.1039/d4nj01136h Kartik Dutta 1 , Saparya Chattaraj 2, 3 , Riddhi Pal 1 , Padma Nilaya Jonnalgadda 2, 4 , Birija S. Patro 1, 2 , Soumyaditya Mula 1, 2 , Goutam Chakraborty 4
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Highly sensitive and easy to use sensors are required to measure in vitro and in vivo sulfite ion concentrations for better human health. To date various fluorescence-based probes have been utilized for sulfite detection, but most of them suffer from cumbersome synthetic protocols and lengthy detection times, which somewhat limit their widespread applications. Herein, we have developed a highly selective and fast responsive fluorescent probe, Kumujian-C (Kum-C) for the detection of sulfite ions in both in vitro and in vivo conditions. Kum-C, a β-carboline-based small molecule fluorescent probe, was synthesised and its photophysical properties in different conditions were evaluated. The aqueous solution of the probe was weakly fluorescent, but upon selective binding with sulfite ions, the probe instantly displayed a phenomenal enhancement in its fluorescence intensity. This turn-on response was found to be linear over the range of 0 to 125 μM sulfite concentration, enabling the probe to detect sulfite down to 0.35 μM in buffer. Moreover, high selectivity, and fast response toward sulfite ions over other anions and bio-analytes, established the developed probe as an efficient sulfite sensor. Finally, using the probe, the detection of sulfite ions in real food samples as well as in living cells has also been demonstrated.
中文翻译：
基于 Kumujian-C 的高选择性荧光开启探针能够检测真实样品和活细胞中的亚硫酸盐
为了改善人类健康，需要高度灵敏且易于使用的传感器来测量体外和体内亚硫酸根离子浓度。迄今为止，各种基于荧光的探针已用于亚硫酸盐检测，但大多数都存在繁琐的合成方案和漫长的检测时间，这在一定程度上限制了它们的广泛应用。在此，我们开发了一种高选择性和快速响应的荧光探针 Kumujian-C (Kum-C)，用于在体外和体内条件下检测亚硫酸根离子。合成了基于β-咔啉的小分子荧光探针Kum-C，并评估了其在不同条件下的光物理性质。探针的水溶液发出微弱的荧光，但在与亚硫酸根离子选择性结合后，探针的荧光强度立即显着增强。发现这种开启响应在亚硫酸盐浓度 0 至 125 μM 范围内呈线性，使探针能够检测缓冲液中低至 0.35 μM 的亚硫酸盐。此外，与其他阴离子和生物分析物相比，对亚硫酸盐离子的高选择性和快速响应使所开发的探针成为高效的亚硫酸盐传感器。最后，还证明了使用该探针可以检测真实食品样品以及活细胞中的亚硫酸根离子。
更新日期：2024-05-03
中文翻译：
基于 Kumujian-C 的高选择性荧光开启探针能够检测真实样品和活细胞中的亚硫酸盐
为了改善人类健康，需要高度灵敏且易于使用的传感器来测量体外和体内亚硫酸根离子浓度。迄今为止，各种基于荧光的探针已用于亚硫酸盐检测，但大多数都存在繁琐的合成方案和漫长的检测时间，这在一定程度上限制了它们的广泛应用。在此，我们开发了一种高选择性和快速响应的荧光探针 Kumujian-C (Kum-C)，用于在体外和体内条件下检测亚硫酸根离子。合成了基于β-咔啉的小分子荧光探针Kum-C，并评估了其在不同条件下的光物理性质。探针的水溶液发出微弱的荧光，但在与亚硫酸根离子选择性结合后，探针的荧光强度立即显着增强。发现这种开启响应在亚硫酸盐浓度 0 至 125 μM 范围内呈线性，使探针能够检测缓冲液中低至 0.35 μM 的亚硫酸盐。此外，与其他阴离子和生物分析物相比，对亚硫酸盐离子的高选择性和快速响应使所开发的探针成为高效的亚硫酸盐传感器。最后，还证明了使用该探针可以检测真实食品样品以及活细胞中的亚硫酸根离子。
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