Perivascular collagen deposition by activated fibroblasts promotes vascular stiffening and drives cardiovascular diseases such as pulmonary hypertension (PH). Whether and how vascular fibroblasts rewire their metabolism to sustain collagen biosynthesis remains unknown. Here, we found that inflammation, hypoxia, and mechanical stress converge on activating the transcriptional coactivators YAP and TAZ (WWTR1) in pulmonary arterial adventitial fibroblasts (PAAFs). Consequently, YAP and TAZ drive glutamine and serine catabolism to sustain proline and glycine anabolism and promote collagen biosynthesis. Pharmacologic or dietary intervention on proline and glycine anabolic demand decreases vascular stiffening and improves cardiovascular function in PH rodent models. By identifying the limiting metabolic pathways for vascular collagen biosynthesis, our findings provide guidance for incorporating metabolic and dietary interventions for treating cardiopulmonary vascular disease.

中文翻译：

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膳食摄入和谷氨酰胺-丝氨酸代谢控制病理性血管僵硬度


活化的成纤维细胞引起的血管周围胶原蛋白沉积会促进血管硬化并引发肺动脉高压（PH）等心血管疾病。血管成纤维细胞是否以及如何重新连接其新陈代谢以维持胶原蛋白生物合成仍然未知。在这里，我们发现炎症、缺氧和机械应力共同激活肺动脉外膜成纤维细胞（PAAF）中的转录共激活因子 YAP 和 TAZ (WWTR1)。因此，YAP 和 TAZ 驱动谷氨酰胺和丝氨酸分解代谢，以维持脯氨酸和甘氨酸合成代谢并促进胶原蛋白生物合成。在 PH 啮齿动物模型中，对脯氨酸和甘氨酸合成代谢需求进行药物或饮食干预可减少血管硬化并改善心血管功能。通过确定血管胶原生物合成的限制代谢途径，我们的研究结果为结合代谢和饮食干预治疗心肺血管疾病提供了指导。