Cell Research ( IF 28.1 ) Pub Date : 2024-04-30 , DOI: 10.1038/s41422-024-00966-9 Aleksey Raskovalov 1, 2 , Donggyun Kim 1, 2 , Vadim Cherezov 1, 2
G protein-coupled receptors (GPCRs) represent some of the most important drug targets in the human genome, however, our understanding of these receptors has primarily been confined to systems that require the overexpression of target receptors. In a recent study published in Cell, Janicot et al. introduced a universal biosensor platform capable of detecting GPCR signaling at endogenous levels, which facilitates its application across various cell lines, including primary cells, thereby improving our understanding of context-dependent GPCR activity and system bias.
中文翻译:
ONE-GO:直接检测上下文相关的 GPCR 活性
G蛋白偶联受体(GPCR)代表了人类基因组中一些最重要的药物靶标,然而,我们对这些受体的理解主要局限于需要靶受体过度表达的系统。 Janicot 等人最近发表在《细胞》杂志上的一项研究中。推出了一种能够在内源水平检测 GPCR 信号传导的通用生物传感器平台,这有助于其在包括原代细胞在内的各种细胞系中的应用,从而提高我们对背景依赖性 GPCR 活性和系统偏差的理解。