Antibiotic-associated diarrhea is a common adverse reaction caused by the widespread use of antibiotics. The decrease in probiotics is one of the reasons why antibiotics cause drug-induced diarrhea. However, few studies have addressed the intrinsic mechanism of antibiotics inhibiting probiotics. To investigate the underlying mechanism of levofloxacin against Bifidobacterium adolescentis, we used a metabolomics mass spectrometry-based approach and molecular docking analysis for a levofloxacin-induced B. adolescentis injury model. The results showed that levofloxacin reduced the survival rate of B. adolescentis and decreased the number of B. adolescentis. The untargeted metabolomics analysis identified 27 potential biomarkers, and many of these metabolites are involved in energy metabolism, amino acid metabolism and the lipid metabolism pathway. Molecular docking showed that levofloxacin can bind with aminoacyl-tRNA synthetase and lactic acid dehydrogenase. This result provides a novel insight into the mechanism of the adverse reactions of levofloxacin.

中文翻译：

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基于 UPLC/Q-TOF-MS 的青少年双歧杆菌左氧氟沙星暴露代谢组学和分子对接分析


抗生素相关性腹泻是广泛使用抗生素引起的常见不良反应。益生菌的减少是抗生素引起药物性腹泻的原因之一。然而，很少有研究探讨抗生素抑制益生菌的内在机制。为了研究左氧氟沙星对抗青春双歧杆菌的潜在机制，我们使用基于代谢组学质谱的方法和分子对接分析来建立左氧氟沙星诱导的青春双歧杆菌损伤模型。结果表明，左氧氟沙星降低了青春双歧杆菌的存活率，并减少了青春双歧杆菌的数量。非靶向代谢组学分析确定了 27 个潜在的生物标志物，其中许多代谢物涉及能量代谢、氨基酸代谢和脂质代谢途径。分子对接表明，左氧氟沙星可以与氨酰-tRNA合成酶和乳酸脱氢酶结合。这一结果为左氧氟沙星不良反应的机制提供了新的见解。