Three types of enzymes complete the furanocoumarins core skeleton biosynthesis in Angelica sinensis,Phytochemistry

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Three types of enzymes complete the furanocoumarins core skeleton biosynthesis in Angelica sinensis
Phytochemistry ( IF 3.2 ) Pub Date : 2024-04-18 , DOI: 10.1016/j.phytochem.2024.114102
Kaixuan Wang ₁ , Huihui Zeng ₁ , Yiqun Dai ₂ , Zixuan Wang ₁ , Huanying Tang ₁ , Junde Li ₃ , Xingchen Lu ₁ , Neng Jiang ₄ , Guoyong Xie ₁ , Yan Zhu ₁ , Yucheng Zhao ₅ , Minjian Qin ₅

Affiliation

Furanocoumarins (FCs) are widely distributed secondary metabolites found in higher plants, including Apiaceae, Rutaceae, Moraceae, and Fabaceae. They play a crucial role in the physiological functions of plants and are well-known for their diverse pharmacological activities. As a representative plant of the Apiaceae family, is highly valued for its medicinal properties and FCs are one of the main ingredients of . However, the biosynthetic mechanism of FCs in remains poorly understood. In this study, we successfully cloned and verified three types of enzymes using genome analysis and functional verification, which complete the biosynthesis of the FCs core skeleton in . It includes a -coumaroyl CoA 2′-hydroxylase () responsible for umbelliferone formation, two UbiA prenyltransferases ( and ) that convert umbelliferone to demethylsuberosin (DMS) and osthenol, respectively, and two CYP736 subfamily cyclases ( and ) that catalyze the formation of FCs core skeleton. Interestingly, was demonstrated to be a bifunctional cyclase and could catalyze both DMS and osthenol, but had a higher affinity to osthenol. The characterization of these enzymes elucidates the molecular mechanism of FCs biosynthesis, providing new insights and technologies for understanding the diverse origins of FCs biosynthesis.

中文翻译：

三种酶完成当归中呋喃香豆素核心骨架的生物合成

呋喃香豆素（FCs）是广泛存在于高等植物中的次生代谢产物，包括伞形科、芸香科、桑科和豆科植物。它们在植物的生理功能中发挥着至关重要的作用，并以其多样化的药理活性而闻名。作为伞形科植物的代表植物，其药用特性备受重视，FCs是伞形花的主要成分之一。然而，FCs 的生物合成机制仍然知之甚少。本研究通过基因组分析和功能验证，成功克隆并验证了三类酶，完成了FCs核心骨架的生物合成。它包括负责伞形酮形成的 -香豆酰辅酶 A 2'-羟化酶 ()、分别将伞形酮转化为去甲基木香皂素 (DMS) 和 osthenol 的两种 UbiA 异戊烯基转移酶 ( 和 )，以及催化 FC 形成的两种 CYP736 亚家族环化酶 ( 和 )核心骨架。有趣的是，它被证明是一种双功能环化酶，可以催化 DMS 和 osthenol，但对 osthenol 具有更高的亲和力。这些酶的表征阐明了FCs生物合成的分子机制，为理解FCs生物合成的不同起源提供了新的见解和技术。

更新日期：2024-04-18

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