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Optical Fiber Surface Plasmon Resonance Sensor for Glyceryl Tributyrate Detection Based on the PAA/CS Composite Hydrogel Embedding Protease Method
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-04-24 , DOI: 10.1021/acs.analchem.3c05357 Hongxin Zhang 1 , Xuegang Li 1, 2 , Xue Zhou 1, 2 , Yanan Zhang 1 , Yong Zhao 1, 3
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-04-24 , DOI: 10.1021/acs.analchem.3c05357 Hongxin Zhang 1 , Xuegang Li 1, 2 , Xue Zhou 1, 2 , Yanan Zhang 1 , Yong Zhao 1, 3
Affiliation
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Glycerol tributyrate as a low-density lipoprotein plays a crucial role in drug development and food safety. In this work, a novel high-stability fiber optic sensor for glyceryl tributyrate based on the poly(acrylic acid) (PAA) and chitosan (CS) composite hydrogel embedding method is first proposed. Compared with traditional functionalization, the lipase in a polymer network structure used in this article can not only avoid chemical reactions that cause damage to the enzyme structure but also avoid the instability of ionic bonds and physical adsorption. Therefore, the PAA/CS hydrogel method proposed in this article can effectively retain enzyme structure. First, the impact of different layers (one to five layers) of PAA/CS on pH sensing performance was explored, and it was determined that layers 1–3 could be used for subsequent sensing experiments. Within the linear detection range of 0.5–10 mM, the detection sensitivities of the one to three layers of the biosensor are divided into 0.65, 0.95, and 1.51 nm/mM, respectively, with the three layers having the best effect. When the number of coating layers is three, the detection limit of the sensor is 0.47 mM, meeting the millimole level detection standard for anticancer requirement. Furthermore, the stability and selectivity of the sensor (in the presence of hemoglobin, urea, cholesterol, acetylcholine, and glucose) were analyzed. The three-layer sensor is used for sample detection. At concentrations of 1–10 mM, the absolute value of the recovery percentage (%) is 82–99%, which can accurately detect samples. The sensor proposed in this paper has the advantages of low sample consumption, high sensitivity, simple structure, and label-free measurement. The enzyme-embedding method provides a new route for rapid and reliable glyceryl tributyrate detection, which has potential applications in food safety as well as the development of anticancer drugs.
中文翻译：
基于PAA/CS复合水凝胶包埋蛋白酶法检测甘油三丁酸的光纤表面等离子体共振传感器
三丁酸甘油酯作为一种低密度脂蛋白，在药物开发和食品安全中发挥着至关重要的作用。在这项工作中，首次提出了一种基于聚丙烯酸（PAA）和壳聚糖（CS）复合水凝胶嵌入方法的新型高稳定性三丁酸甘油酯光纤传感器。与传统的功能化相比，本文采用的聚合物网络结构的脂肪酶不仅可以避免化学反应对酶结构造成破坏，而且可以避免离子键和物理吸附的不稳定性。因此，本文提出的PAA/CS水凝胶方法可以有效保留酶结构。首先，探讨了PAA/CS不同层数（一到五层）对pH传感性能的影响，并确定第1-3层可用于后续传感实验。在0.5~10 mM的线性检测范围内，生物传感器的一到三层的检测灵敏度分别分为0.65、0.95和1.51 nm/mM，其中三层效果最好。当涂层数为三层时，传感器的检测限为0.47 mM，满足抗癌要求的毫摩尔级检测标准。此外，还分析了传感器的稳定性和选择性（在存在血红蛋白、尿素、胆固醇、乙酰胆碱和葡萄糖的情况下）。三层传感器用于样品检测。在1~10 mM浓度下，回收率绝对值为82~99%，可以准确检测样品。本文提出的传感器具有样品消耗少、灵敏度高、结构简单、无需标记测量等优点。 酶包埋法为快速可靠的三丁酸甘油酯检测提供了一条新途径，在食品安全以及抗癌药物的开发方面具有潜在的应用前景。
更新日期：2024-04-24
中文翻译：
基于PAA/CS复合水凝胶包埋蛋白酶法检测甘油三丁酸的光纤表面等离子体共振传感器
三丁酸甘油酯作为一种低密度脂蛋白，在药物开发和食品安全中发挥着至关重要的作用。在这项工作中，首次提出了一种基于聚丙烯酸（PAA）和壳聚糖（CS）复合水凝胶嵌入方法的新型高稳定性三丁酸甘油酯光纤传感器。与传统的功能化相比，本文采用的聚合物网络结构的脂肪酶不仅可以避免化学反应对酶结构造成破坏，而且可以避免离子键和物理吸附的不稳定性。因此，本文提出的PAA/CS水凝胶方法可以有效保留酶结构。首先，探讨了PAA/CS不同层数（一到五层）对pH传感性能的影响，并确定第1-3层可用于后续传感实验。在0.5~10 mM的线性检测范围内，生物传感器的一到三层的检测灵敏度分别分为0.65、0.95和1.51 nm/mM，其中三层效果最好。当涂层数为三层时，传感器的检测限为0.47 mM，满足抗癌要求的毫摩尔级检测标准。此外，还分析了传感器的稳定性和选择性（在存在血红蛋白、尿素、胆固醇、乙酰胆碱和葡萄糖的情况下）。三层传感器用于样品检测。在1~10 mM浓度下，回收率绝对值为82~99%，可以准确检测样品。本文提出的传感器具有样品消耗少、灵敏度高、结构简单、无需标记测量等优点。 酶包埋法为快速可靠的三丁酸甘油酯检测提供了一条新途径，在食品安全以及抗癌药物的开发方面具有潜在的应用前景。
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