Ancestral TALE homeobox protein transcription factor regulates actin dynamics and cellular activities of protozoan parasite Entamoeba invadens,Molecular Microbiology

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Ancestral TALE homeobox protein transcription factor regulates actin dynamics and cellular activities of protozoan parasite Entamoeba invadens
Molecular Microbiology ( IF 2.6 ) Pub Date : 2024-04-23 , DOI: 10.1111/mmi.15266
Meenakshi Pandey ₁ , Shilpa Sarkar ₁ , Sudip K Ghosh ₁

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Entamoeba histolytica causes invasive amoebiasis, an important neglected tropical disease with a significant global health impact. The pathogenicity and survival of E. histolytica and its reptilian equivalent, Entamoeba invadens, relies on its ability to exhibit efficient motility, evade host immune responses, and exploit host resources, all of which are governed by the actin cytoskeleton remodeling. Our study demonstrates the early origin and the regulatory role of TALE homeobox protein EiHbox1 in actin-related cellular processes. Several genes involved in different biological pathways, including actin dynamics are differentially expressed in EiHbox1 silenced cells. EiHbox1 silenced parasites showed disrupted F-actin organization and loss of cellular polarity. EiHbox1's presence in the anterior region of migrating cells further suggests its involvement in maintaining cellular polarity. Loss of polarized morphology of EiHbox1 silenced parasites leads to altered motility from fast, directionally persistent, and highly chemotactic to slow, random, and less chemotactic, which subsequently leads to defective aggregation during encystation. EiHbox1 knockdown also resulted in a significant reduction in phagocytic capacity and poor capping response. These findings highlight the importance of EiHbox1 of E. invadens in governing cellular processes crucial for their survival, pathogenicity, and evasion of the host immune system.

中文翻译：

祖先 TALE 同源框蛋白转录因子调节原生动物寄生虫内阿米巴入侵的肌动蛋白动力学和细胞活性

溶组织内阿米巴可引起侵袭性阿米巴病，这是一种重要的被忽视的热带病，对全球健康有重大影响。溶组织肠球菌及其爬行动物等价物内阿米巴入侵的致病性和存活率取决于其表现出有效运动、逃避宿主免疫反应和利用宿主资源的能力，所有这些都受肌动蛋白细胞骨架重塑的控制。我们的研究证明了 TALE 同源盒蛋白 EiHbox1 的早期起源和在肌动蛋白相关细胞过程中的调节作用。参与不同生物途径（包括肌动蛋白动力学）的几个基因在 EiHbox1 沉默细胞中差异表达。EiHbox1 沉默的寄生虫显示 F-肌动蛋白组织中断和细胞极性丧失。EiHbox1 存在于迁移细胞的前部区域，进一步表明它参与维持细胞极性。EiHbox1 沉默寄生虫的极化形态缺失导致运动从快速、方向持续和高度趋化性改变为缓慢、随机和较少趋化，随后导致包扎期间聚集缺陷。EiHbox1 敲低还导致吞噬能力显著降低和加帽反应不良。这些发现强调了 E. invadens 的 EiHbox1 在控制细胞过程中的重要性，这些细胞过程对其生存、致病性和逃避宿主免疫系统至关重要。

更新日期：2024-04-23

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