This study investigates the creation and analysis of chitosan-zinc oxide (CS-ZnO) nanocomposites, exploring their effectiveness in inhibiting bacteria. Two synthesis approaches, physical and chemical, were utilized. The CS-ZnO nanocomposites demonstrated strong antibacterial properties, especially against Staphylococcus aureus, a Gram-positive bacterium. Chemically synthesized nanocomposites (CZ10 and CZ100) exhibited larger inhibition zones (16.4 mm and 18.7 mm) compared to physically prepared CS-Z5 and CS-Z20 (12.2 mm and 13.8 mm) against Staphylococcus aureus. Moreover, CZ nanocomposites displayed enhanced thermal stability, with decomposition temperatures of 281°C and 290°C, surpassing CS-Z5 and CS-Z20 (260°C and 258°C). The residual mass percentages at 800°C were significantly higher for CZ10 and CZ100 (58% and 61%) than for CS-Z5 and CS-Z20 (36% and 34%). UV–Visible spectroscopy revealed reduced band gaps in the CS-ZnO nanocomposites, indicating improved light absorption. Transmission electron microscopy (TEM) confirmed uniform dispersion of ZnO nanoparticles within the chitosan matrix. In conclusion, this research underscores the impressive antimicrobial potential of CS-ZnO nanocomposites, especially against Gram-positive bacteria, and highlights their enhanced thermal stability. These findings hold promise for diverse applications in industries such as medicine, pharmaceuticals, and materials science, contributing to the development of sustainable materials with robust antimicrobial properties.

本研究研究了壳聚糖-氧化锌 (CS-ZnO) 纳米复合材料的制备和分析，探索其抑制细菌的有效性。使用物理和化学两种合成方法。 CS-ZnO纳米复合材料表现出强大的抗菌性能，特别是针对金黄色葡萄球菌（一种革兰氏阳性细菌）。与物理制备的CS-Z5和CS-Z20（12.2毫米和13.8毫米）相比，化学合成的纳米复合材料（CZ10和CZ100）对金黄色葡萄球菌表现出更大的抑制区（16.4毫米和18.7毫米） 。此外，CZ纳米复合材料表现出增强的热稳定性，分解温度为281°C和290°C，超过CS-Z5和CS-Z20（260°C和258°C）。 CZ10 和 CZ100 在 800°C 时的残余质量百分比（58% 和 61%）显着高于 CS-Z5 和 CS-Z20（36% 和 34%）。紫外-可见光谱显示 CS-ZnO 纳米复合材料的带隙减小，表明光吸收得到改善。透射电子显微镜 (TEM) 证实 ZnO 纳米粒子均匀分散在壳聚糖基质内。总之，这项研究强调了 CS-ZnO 纳米复合材料令人印象深刻的抗菌潜力，特别是针对革兰氏阳性菌，并强调了它们增强的热稳定性。这些发现为医学、制药和材料科学等行业的多种应用带来了希望，有助于开发具有强大抗菌性能的可持续材料。