Mechanisms on the removal of gram-negative/positive antibiotic resistant bacteria and inhibition of horizontal gene transfer by ferrate coupled with peroxydisulfate or peroxymonosulfate,Journal of Hazardous Materials

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Mechanisms on the removal of gram-negative/positive antibiotic resistant bacteria and inhibition of horizontal gene transfer by ferrate coupled with peroxydisulfate or peroxymonosulfate
Journal of Hazardous Materials ( IF 12.2 ) Pub Date : 2024-04-10 , DOI: 10.1016/j.jhazmat.2024.134254
Xiaoyu Fu ₁ , Jingfeng Gao ₁ , Qian Wang ₁ , Hao Chen ₁ , Ying Liu ₁ , Liqin Zeng ₁ , Yukun Yuan ₁ , Hongxin Xu ₁

Affiliation

The existence of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) has been a global public environment and health issue. Due to the different cell structures, gram-positive/negative ARB exhibit various inactivation mechanisms in water disinfection. In this study, a gram-negative ARB DH5α ( DH5α) was used as a horizontal gene transfer (HGT) donor, while a gram-positive ARB as a recipient. To develop an efficient and engineering applicable method in water disinfection, ARB and ARGs removal efficiency of Fe(VI) coupled peroxydisulfate (PDS) or peroxymonosulfate (PMS) was compared, wherein hydroxylamine (HA) was added as a reducing agent. The results indicated that Fe(VI)/PMS/HA showed higher disinfection efficiency than Fe(VI)/PDS/HA. When the concentration of each Fe(VI), PMS, HA was 0.48 mM, 5.15 log DH5α and 3.57 log lost cultivability, while the proportion of recovered cells was 0.0017 % and 0.0566 %, respectively, and HGT was blocked. Intracellular was reduced by 2.49 log. Fe(IV) and/or Fe(V) were proved to be the decisive reactive species. Due to the superiority of low cost as well as high efficiency and practicality, Fe(VI)/PMS/HA has significant application potential in ARB, ARGs removal and HGT inhibition, offering a new insight for wastewater treatment.

中文翻译：

铁酸盐与过氧二硫酸盐或过氧一硫酸盐偶联对革兰氏阴性/阳性抗生素耐药菌的去除和抑制水平基因转移的机制

抗生素耐药菌（ARB）和抗生素耐药基因（ARG）的存在一直是全球公共环境和健康问题。由于细胞结构不同，革兰氏阳性/阴性 ARB 在水消毒中表现出不同的灭活机制。在本研究中，革兰氏阴性 ARB DH5α （ DH5α）用作水平基因转移（HGT）供体，而革兰氏阳性 ARB 作为受体。为了开发一种高效且工程适用的水消毒方法，比较了 Fe（VI）偶联过氧二硫酸盐（PDS）或过氧一硫酸盐（PMS）的 ARB 和 ARGs 去除效率，其中添加羟胺（HA）作为还原剂。结果表明，Fe（VI）/PMS/HA 的消毒效率高于 Fe（VI）/PDS/HA。当 Fe（VI）、PMS、HA 浓度为 0.48 mM 时，DH5α 和 3.57 log 丧失培养性，而回收细胞比例分别为 0.0017 % 和 0.0566 %，HGT 被阻断。细胞内减少 2.49 log。Fe（IV）和/或 Fe（V）被证明是决定性的反应性物质。Fe（VI）/PMS/HA 具有成本低、高效实用等优点，在 ARB、ARGs 去除和 HGT 抑制方面具有显著的应用潜力，为废水处理提供了新的思路。

更新日期：2024-04-10

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