<br>基于新型DNA依赖性PBNF@PtPd的荧光电化学双模式适体传感器，通过杂交链式反应高选择性和灵敏地检测腐霉利,Science of the Total Environment

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基于新型DNA依赖性PBNF@PtPd的荧光电化学双模式适体传感器，通过杂交链式反应高选择性和灵敏地检测腐霉利
Science of the Total Environment ( IF 8.2 ) Pub Date : 2024-04-15 , DOI: 10.1016/j.scitotenv.2024.172529
Ruonan Wang ₁ , Baoshan He ₁ , Jun Yang ₂ , Yao Liu ₃ , Zhengyong Liang ₄ , Huali Jin ₁ , Min Wei ₁ , Wenjie Ren ₁ , Zhiguang Suo ₁ , Yiwei Xu ₁

Affiliation

在此，一项研究首次应用杂交链式反应、1,8-萘二甲酰亚胺-DNA (NDs) 嵌入剂和 DNA 依赖性普鲁士蓝纳米花@PtPd 材料 (PBNFs@PtPd) 在荧光电化学开发中的应用(FL-EC)适体传感器。该结构建立了一个用于检测腐霉利（PCM）的高效传感平台。在所述实验的背景下，双模式检测是通过用 Cy5 部分标记的适体产生 FL 信号以及通过修饰硫堇附加的 1,8-萘二甲酰亚胺 (Thi- ND）。在 PCM 存在的情况下，发生特异性识别，然后利用磁分离技术释放 DNA1 (S1) 和适体-Cy5 (Apt-Cy5)，随后将它们引入荧光和 EC 平台。 S1的存在有效激活了电极表面的杂交链式反应（HCR），从而显着增加了Thi-ND的结合位点，从而大大放大了差分脉冲伏安法（DPV）的响应信号。所开发的FL-EC双模式传感平台对PCM的检测具有高灵敏度，检测限为0.173 μg·ml（在500 pg·ml至500 ng·ml的检测范围内）和0.074 ng·ml（在500 pg·ml至500 ng·ml的检测范围内）。分别在100 pg·ml至100 ng·ml的检测范围内）。设计的双模式传感器具有显着的特性，包括高选择性、再现性、协同性以及对实际样品中 PCM 的可靠监测/能力。

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A fluorescence-electrochemical dual-mode aptasensor based on novel DNA-dependent PBNFs@PtPd for highly selective and sensitive detection of procymidone through hybridization chain reaction

Herein, a study for the first application of a hybridization chain reaction, a 1,8-naphthalimides-DNA (NDs) intercalator, and DNA-dependent Prussian blue nanoflowers@PtPd materials (PBNFs@PtPd) in the development of a fluorescence-electrochemical (FL-EC) aptasensor. This construction establishes an efficient sensing platform for the detection of procymidone (PCM). In the context of the described experiment, dual-mode detection is achieved through the generation of FL signals by an aptamer labeled with a Cy5 moiety and the formation of DPV signals by the modification of a thionine-appended 1,8-naphthalimide (Thi-NDs). In the presence of PCM, specific recognition occurs, followed by the utilization of magnetic separation technology to release DNA1 (S1) and aptamer-Cy5 (Apt-Cy5), subsequently introducing them onto both fluorescence and EC platforms. The presence of S1 effectively activates hybridization chain reaction (HCR) for the electrode surface, thereby significantly increasing the binding sites for Thi-NDs and consequently greatly amplifying the response signal of differential pulse voltammetry (DPV). The developed FL-EC dual-mode sensing platform demonstrates high sensitivity in the detection of PCM, with the detection limits of 0.173 μg·ml (within the detection range of 500 pg·ml to 500 ng·ml) and 0.074 ng·ml (within the detection range of 100 pg·ml to 100 ng·ml), respectively. The designed dual-mode sensor exhibits notable characteristics, including high selectivity, reproducibility, synergy, and reliable monitoring/capability for PCM in real samples.

更新日期：2024-04-15

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