<br>JOINTLESS 保持番茄花序分生组织的同一性,Plant & Cell Physiology

当前位置： X-MOL 学术 › Plant Cell Physiol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

JOINTLESS 保持番茄花序分生组织的同一性
Plant & Cell Physiology ( IF 3.9 ) Pub Date : 2024-04-17 , DOI: 10.1093/pcp/pcae046
Samuel Huerga-Fernández ₁ , Nathalie Detry ₁ , Beata Orman-Ligeza ₁ , Frédéric Bouché _{1,

2} , Marc Hanikenne ₂ , Claire Périlleux ₁

Affiliation

JOINTLESS (J) 是在番茄 (Solanum lycopersicum) 中从缺乏花梗脱落区 (AZ) 的突变体中分离出来的，它编码 SVP/AGL24 亚家族的 MADS-box 蛋白。 J 功能的丧失也会导致花序中叶起始的恢复，表明在花序分生组织特性中起着关键作用。在这里，我们比较了不同种质中的 j 突变体，这些突变体要么表现出不确定的芽生长，产生规则的丛生节段，要么表现出确定的芽生长，这是由于丛生节段的减少和自修剪（SP）基因的因果突变。我们观察到 j 突变体的花序表型在不确定 (SP) 种质（例如 Ailsa Craig (AC)）中比在确定 (sp) 种质（例如 Heinz (Hz)）中更强。此外，RNA-seq分析表明，j突变体恢复营养命运伴随着SP的表达，SP支持j花序中花序分生组织向丛生芽分生组织的转化。营养分生组织的其他标记（例如 APETALA2c）和分支基因（例如 BRANCHED 1 (BRC1a/b)）在 j(AC) 突变体的花序中差异表达。我们还发现，在不确定的 AC 种质中，J 抑制 B 类和 C 类的同源异型基因，并且其过度表达会导致过大的叶状花萼表型，并对 AZ 形成具有显性负面影响。因此提出了一个模型，其中 J 通过抑制芽命运和影响生殖器官形成，充当花序分生组织的关键决定因素。

"点击查看英文标题和摘要"

JOINTLESS Maintains Inflorescence Meristem Identity in Tomato

JOINTLESS (J) was isolated in tomato (Solanum lycopersicum) from mutants lacking a flower pedicel abscission zone (AZ), and encodes a MADS-box protein of the SVP/AGL24 sub-family. The loss of J function also causes the return to leaf initiation in the inflorescences, indicating a pivotal role in inflorescence meristem identity. Here, we compared j mutants in different accessions that exhibit either an indeterminate shoot growth, producing regular sympodial segments, or a determinate shoot growth, due to the reduction of sympodial segments and causal mutation of the SELF PRUNING (SP) gene. We observed that the inflorescence phenotype of j mutants is stronger in indeterminate (SP) accessions such as Ailsa Craig (AC), than in determinate (sp) ones, such as Heinz (Hz). Moreover, RNA-seq analysis revealed that the return to vegetative fate in j mutants is accompanied by expression of SP, which supports conversion of the inflorescence meristem to sympodial shoot meristem in j inflorescences. Other markers of vegetative meristems such as APETALA2c, and branching genes such as BRANCHED 1 (BRC1a/b) were differentially expressed in the inflorescences of j(AC) mutants. We also found in the indeterminate AC accession that J represses homeotic genes of B- and C-classes, and that its overexpression causes an oversized leafy calyx phenotype and has a dominant negative effect on AZ formation. A model is therefore proposed where J, by repressing shoot fate and influencing reproductive organ formation, acts as a key determinant of inflorescence meristems.

更新日期：2024-04-17

点击分享查看原文

点击收藏

公开下载

阅读更多本刊最新论文