The large-scale cultivation of photosynthetic bacteria requires significant quantities of fresh water. Screening strains that can thrive in brackish water or seawater is a solution to mitigate the dwindling availability of freshwater. Through phylogenetic analysis based on 16S rDNA sequences, this study identified a strain MBE isolated from seawater. The effects of several key factors, including carbon sources, pH, NH and NaCl concentration on hydrogen production performance were investigated. MBE showed a preference for glucose, but not organic acids for hydrogen production. It also exhibited alkalinity, achieving its peak hydrogen yield of 4626.82 ± 155.65 mL⋅L at pH 8. Interestingly, MBE could exploit ammonium nitrogen for hydrogen synthesis, thriving even in specific ammonium and salt concentrations. Its resilience was further highlighted by a maximum hydrogen production yield of 4741.41 ± 167.01 mL⋅L and a hydrogen production rate of 37.59 ± 3.37 mL⋅L⋅h with 10 g⋅L NaCl, underscoring its significant salt tolerance. These results suggested that MBE could be a promising candidate for biofuel production, and it is significant to take sustainability study on the application of MBE to deal with alkali wastewater from paper making, pharmacy and printing and high salinity wastewater in the future.

中文翻译：

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一种新分离的耐盐凝胶红红霉MBE，能够在铵存在下产生氢气

光合细菌的大规模培养需要大量的淡水。筛选可以在咸水或海水中繁殖的菌株是缓解淡水供应减少的一种解决方案。本研究通过基于16S rDNA序列的系统发育分析，鉴定出一株从海水中分离的MBE菌株。研究了碳源、pH、NH 和 NaCl 浓度等几个关键因素对制氢性能的影响。 MBE 显示出偏爱葡萄糖，但不偏爱有机酸来产生氢气。它还表现出碱性，在 pH 8 时达到 4626.82 ± 155.65 mL·L 的峰值氢产率。有趣的是，MBE 可以利用铵氮进行氢合成，即使在特定的铵和盐浓度下也能蓬勃发展。其恢复能力进一步突出，最大产氢量为 4741.41 ± 167.01 mL·L，在 10 g·L NaCl 下的产氢率为 37.59 ± 3.37 mL·L·h，强调了其显着的耐盐性。这些结果表明MBE可能是生物燃料生产的一个有前途的候选者，并且对未来应用MBE处理造纸、制药和印刷的碱性废水以及高盐废水进行可持续性研究具有重要意义。