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Metabolism of Inulin via Difructose Anhydride I Pathway in Microbacterium flavum
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-04-17 , DOI: 10.1021/acs.jafc.4c00729 Qiting Li 1 , Zhenlong Wang 1 , Mengyan Zhu 1 , Wei Zhao 1 , Shuhuai Yu 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-04-17 , DOI: 10.1021/acs.jafc.4c00729 Qiting Li 1 , Zhenlong Wang 1 , Mengyan Zhu 1 , Wei Zhao 1 , Shuhuai Yu 1
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Difructose anhydride I (DFA-I) can be produced from inulin, with DFA-I-forming inulin fructotransferase (IFTase-I). However, the metabolism of inulin through DFA-I remains unclear. To clarify this pathway, several genes of enzymes related to this pathway in the genome of Microbacterium flavum DSM 18909 were synthesized, and the corresponding enzymes were encoded, purified, and investigated in vitro. After inulin is decomposed to DFA-I by IFTase-I, DFA-I is hydrolyzed to inulobiose by DFA-I hydrolase. Inulobiose is then hydrolyzed by β-fructofuranosidase to form fructose. Finally, fructose enters glycolysis through fructokinase. A β-fructofuranosidase (MfFFase1) clears the byproducts (sucrose and fructo-oligosaccharides), which might be partially hydrolyzed by fructan β-(2,1)-fructosidase/1-exohydrolase and another fructofuranosidase (MfFFase2). Exploring the DFA-I pathway of inulin and well-studied enzymes in vitro extends our basic scientific knowledge of the energy-providing way of inulin, thereby paving the way for further investigations in vivo and offering a reference for further nutritional investigation of inulin and DFA-I in the future.
中文翻译：
黄色微杆菌中菊粉通过二果糖酐 I 途径的代谢
二果糖酐 I (DFA-I) 可以由菊粉通过形成 DFA-I 的菊粉果糖转移酶 (IFTase-I) 生产。然而，菊粉通过 DFA-I 的代谢仍不清楚。为了阐明该途径，合成了黄色微杆菌DSM 18909基因组中与该途径相关的几个酶基因，并对相应的酶进行了编码、纯化和体外研究。菊粉被IFTase-I分解为DFA-I后，DFA-I被DFA-I水解酶水解为菊粉二糖。然后菊二糖被 β-呋喃果糖苷酶水解形成果糖。最后，果糖通过果糖激酶进入糖酵解。 β-呋喃果糖苷酶 (MfFFase1) 清除副产物（蔗糖和低聚果糖），副产物可能被果聚糖 β-(2,1)-果糖苷酶/1-外切水解酶和另一种呋喃果糖苷酶 (MfFFase2) 部分水解。在体外探索菊粉的DFA-I途径和经过深入研究的酶，扩展了我们对菊粉供能方式的基础科学知识，从而为体内进一步研究铺平了道路，并为菊粉和DFA的进一步营养研究提供了参考-我在未来。
更新日期：2024-04-17
中文翻译：
黄色微杆菌中菊粉通过二果糖酐 I 途径的代谢
二果糖酐 I (DFA-I) 可以由菊粉通过形成 DFA-I 的菊粉果糖转移酶 (IFTase-I) 生产。然而，菊粉通过 DFA-I 的代谢仍不清楚。为了阐明该途径，合成了黄色微杆菌DSM 18909基因组中与该途径相关的几个酶基因，并对相应的酶进行了编码、纯化和体外研究。菊粉被IFTase-I分解为DFA-I后，DFA-I被DFA-I水解酶水解为菊粉二糖。然后菊二糖被 β-呋喃果糖苷酶水解形成果糖。最后，果糖通过果糖激酶进入糖酵解。 β-呋喃果糖苷酶 (MfFFase1) 清除副产物（蔗糖和低聚果糖），副产物可能被果聚糖 β-(2,1)-果糖苷酶/1-外切水解酶和另一种呋喃果糖苷酶 (MfFFase2) 部分水解。在体外探索菊粉的DFA-I途径和经过深入研究的酶，扩展了我们对菊粉供能方式的基础科学知识，从而为体内进一步研究铺平了道路，并为菊粉和DFA的进一步营养研究提供了参考-我在未来。
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