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Low-Triggering-Potential and Narrow-Potential-Window Electrochemiluminescence of Silver Nanoclusters for Gene Assay
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-04-17 , DOI: 10.1021/acs.analchem.3c05970 Yaojia Ai 1 , Xuwen Gao 1 , Xiaoxuan Ren 1 , Mengwei Li 1 , Bin Zhang 1 , Guizheng Zou 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-04-17 , DOI: 10.1021/acs.analchem.3c05970 Yaojia Ai 1 , Xuwen Gao 1 , Xiaoxuan Ren 1 , Mengwei Li 1 , Bin Zhang 1 , Guizheng Zou 1
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A low-triggering potential and a narrow-potential window are anticipated to decrease the electrochemical interference and cross talk of electrochemiluminescence (ECL). Herein, by exploiting the low oxidative potential (0.82 V vs Ag/AgCl) of dihydrolipoic acid-capped sliver nanoclusters (DHLA-AgNCs), a coreactant ECL system of DHLA-AgNCs/hydrazine (N2H4) is proposed to achieve efficient and oxidative-reduction ECL with a low-triggering potential of 0.82 V (vs Ag/AgCl) and a narrow-potential window of 0.22 V. The low-triggering-potential and narrow-potential-window nature of ECL can be primarily preserved upon labeling DHLA-AgNCs to probe DNA and immobilizing DHLA-AgNCs onto the Au surface via sandwiched hybridization, which eventually enables a selective ECL strategy for the gene assay at +0.82 V. This gene assay strategy can sensitively determine the gene of human papillomavirus from 10 to 1000 pM with a low limit of detection of 5 pM (S/N = 3) and would open a way to improve the applied ECL bioassay.
中文翻译：
用于基因检测的银纳米团簇低触发电位和窄电位窗口电化学发光
低触发电位和窄电位窗口有望减少电化学发光 (ECL) 的电化学干扰和串扰。在此，通过利用二氢硫辛酸封端的银纳米团簇（DHLA-AgNCs）的低氧化电位（0.82 V vs Ag/AgCl），提出了DHLA-AgNCs/联氨（N 2 H 4 ）的共反应ECL系统以实现高效和氧化还原ECL，具有0.82 V的低触发电位（相对于Ag/AgCl）和0.22 V的窄电位窗口。ECL的低触发电位和窄电位窗口性质可以主要保留在标记 DHLA-AgNC 来探测 DNA，并通过夹心杂交将 DHLA-AgNC 固定到 Au 表面，最终实现 +0.82 V 基因检测的选择性 ECL 策略。该基因检测策略可以从 10 个基因中灵敏地确定人乳头瘤病毒的基因。至 1000 pM，检测下限为 5 pM (S/N = 3)，这将为改进应用的 ECL 生物测定开辟一条途径。
更新日期：2024-04-17
中文翻译：
用于基因检测的银纳米团簇低触发电位和窄电位窗口电化学发光
低触发电位和窄电位窗口有望减少电化学发光 (ECL) 的电化学干扰和串扰。在此，通过利用二氢硫辛酸封端的银纳米团簇（DHLA-AgNCs）的低氧化电位（0.82 V vs Ag/AgCl），提出了DHLA-AgNCs/联氨（N 2 H 4 ）的共反应ECL系统以实现高效和氧化还原ECL，具有0.82 V的低触发电位（相对于Ag/AgCl）和0.22 V的窄电位窗口。ECL的低触发电位和窄电位窗口性质可以主要保留在标记 DHLA-AgNC 来探测 DNA，并通过夹心杂交将 DHLA-AgNC 固定到 Au 表面，最终实现 +0.82 V 基因检测的选择性 ECL 策略。该基因检测策略可以从 10 个基因中灵敏地确定人乳头瘤病毒的基因。至 1000 pM，检测下限为 5 pM (S/N = 3)，这将为改进应用的 ECL 生物测定开辟一条途径。
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