The chiral fungicide furametpyr is widely used in the rice field to control rice sheath blight; however, furametpyr enantiomers are treated as just one compound in traditional achiral analysis, which gives only partial information. An effective chiral analytical method was developed for the resolution and determination of the fungicide furametpyr enantiomers in rice, soil, and water samples. Furametpyr enantiomers were excellently separated and determined on a Chiralpak AD‐H column with n‐hexane/ethanol (90:10, v/v) as mobile phase at a flow rate of 0.8 mL min^‐1 with UV detection at 220 nm. The resolution was up to 8.85. The first eluted enantiomer was (+)‐furametpyr and the second eluted one was (−)‐furametpyr. The effects of mobile‐phase composition and column temperature on the enantioseparation were evaluated. The method was validated for linearity, repeatability, accuracy, limit of detection (LOD), and limit of quantification LOQ. LOD was 2.0 µg kg^‐1 in water, 0.02 mg kg^‐1 in soil, and 0.07 mg kg^‐1 in rice with an LOQ of 6.7 µg kg^‐1 in water, 0.07 mg kg^‐1 in soil, and 0.23 mg kg^‐1 in rice. The average recoveries of the pesticide in all matrices ranged from 73.1 to 101.8% for all fortification levels. The precision values associated with the analytical method, expressed as relative standard deviation (RSD) values, were below 14.0% in all matrices. The methodology was successfully applied for the enantioselective analysis of furametpyr enantiomers in real samples. Chirality 25:904–909, 2013. © 2013 Wiley Periodicals, Inc.

中文翻译：

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高效液相色谱法拆分大米，土壤和水中的手性杀菌剂富拉米特对映体

手性杀菌剂呋喃咪草在稻田中广泛用于防治稻瘟病。然而，在传统的非手性分析中，呋喃咪草对映体仅被视为一种化合物，仅提供部分信息。开发了一种有效的手性分析方法，用于分离和测定稻米，土壤和水样中的杀真菌剂呋喃吡喃对映体。呋吡菌胺的对映体被非常良好地分离，并且确定在Chiralpak AD-H柱，用Ñ正己烷/乙醇（90:10，V / V）作为以0.8mL min的流速流动相^-1在220 nm处有紫外线检测。分辨率高达8.85。第一个洗脱的对映异构体是（+）-呋喃咪草，第二个洗脱的对映异构体是（-）-呋喃甲草酮。评估了流动相组成和柱温对对映体的影响。验证了该方法的线性，可重复性，准确性，检测限（LOD）和定量限LOQ。LOD为2.0微克千克^-1在水中，0.02毫克千克^-1土壤中，和0.07毫克千克^-1在水稻与6.7微克千克的LOQ ^-1在水中，0.07毫克千克^-1土壤中，和0.23毫克公斤^‐1在米饭中。在所有强化水平下，所有基质中农药的平均回收率在73.1％至101.8％之间。在所有矩阵中，与分析方法相关的精度值（表示为相对标准偏差（RSD）值）均低于14.0％。该方法已成功应用于实际样品中呋喃甲吡对映体的对映选择性分析。手性25：904–909，2013年。©2013 Wiley Periodicals，Inc.