Bacterial resistance and excessive inflammation are common issues that hinder wound healing. Antimicrobial peptides (AMPs) offer a promising and versatile antibacterial option compared to traditional antibiotics, with additional anti-inflammatory properties. However, the applications of AMPs are limited by their antimicrobial effects and stability against bacterial degradation. TFNAs are regarded as a promising drug delivery platform that could enhance the antibacterial properties and stability of nanodrugs. Therefore, in this study, a composite hydrogel (HAMA/t-GL13K) was prepared via the photocross-linking method, in which tFNAs carry GL13K. The hydrogel was injectable, biocompatible, and could be instantly photocured. It exhibited broad-spectrum antibacterial and anti-inflammatory properties by inhibiting the expression of inflammatory factors and scavenging ROS. Thereby, the hydrogel inhibited bacterial infection, shortened the wound healing time of skin defects in infected skin full-thickness defect wound models and reduced scarring. The constructed HAMA/tFNA-AMPs hydrogels exhibit the potential for clinical use in treating microbial infections and promoting wound healing.

细菌耐药性和过度炎症是阻碍伤口愈合的常见问题。与传统抗生素相比，抗菌肽 (AMP) 提供了一种有前景且用途广泛的抗菌选择，并具有额外的抗炎特性。然而，AMP 的应用因其抗菌作用和对细菌降解的稳定性而受到限制。 TFNA被认为是一种有前途的药物递送平台，可以增强纳米药物的抗菌特性和稳定性。因此，本研究通过光交联方法制备了复合水凝胶（HAMA/t-GL13K），其中tFNA携带GL13K。该水凝胶可注射、具有生物相容性，并且可以立即光固化。它通过抑制炎症因子的表达和清除ROS而表现出广谱抗菌和抗炎特性。因此，水凝胶抑制细菌感染，缩短感染皮肤全层缺损伤口模型中皮肤缺损的伤口愈合时间并减少疤痕。构建的 HAMA/tFNA-AMPs 水凝胶具有临床用于治疗微生物感染和促进伤口愈合的潜力。