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A two-step method of cyclolinopeptide (linusorb) preparation from flaxseed cake via dry-screening
Food Chemistry ( IF 8.5 ) Pub Date : 2024-04-09 , DOI: 10.1016/j.foodchem.2024.139243 Zi-Zhe Cai 1 , Chen-Xin Xu 1 , Zi-Liang Song 1 , Jun-le Li 1 , Ning Zhang 1 , Jian-Hao Zhao 2 , Yee-Ying Lee 3 , Martin J T Reaney 4 , Fu-Rong Huang 5 , Yong Wang 1
Food Chemistry ( IF 8.5 ) Pub Date : 2024-04-09 , DOI: 10.1016/j.foodchem.2024.139243 Zi-Zhe Cai 1 , Chen-Xin Xu 1 , Zi-Liang Song 1 , Jun-le Li 1 , Ning Zhang 1 , Jian-Hao Zhao 2 , Yee-Ying Lee 3 , Martin J T Reaney 4 , Fu-Rong Huang 5 , Yong Wang 1
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Linusorbs (LO), cyclolinopeptides, are a group of cyclic hydrophobic peptides and considered a valuable by-product of flaxseed oil due to numerous health benefits. Currently applied acetone or methanol extraction could contaminate the feedstocks for further food-grade application. Using flaxseed cake as feedstock, this study established a practical method for preparing LO from pressed cake. Firstly, LO composition of 15 flaxseed cultivars was analyzed. Next, cold-pressed cake was milled and screened mechanically. The kernel and hull fractions were separated based on the disparity of their mechanical strength. Monitored by hyperspectral fluorescence, the LO-enriched kernel fraction separated from cold-pressed flaxseed cake was further used as feedstock for LO production. After ethanol extraction, partition, and precipitation, LOs were extracted from cold-pressed flaxseed cake with a purity of 91.4%. The proposed method could serve as feasible flaxseed cake valorization strategy and enable the preparation of other polar compounds such as flax lignan and mucilage.
中文翻译：
干法筛选亚麻籽饼制备环亚麻肽（linusorb）的两步法
Linusorbs (LO) 是一种环亚麻肽，是一组环状疏水性肽，由于具有多种健康益处，被认为是亚麻籽油的有价值的副产品。目前应用的丙酮或甲醇提取可能会污染进一步食品级应用的原料。本研究以亚麻籽饼为原料，建立了一种从压榨饼中制备亚麻籽油的实用方法。首先，对15个亚麻籽品种的LO组成进行了分析。接下来，将冷榨饼进行机械研磨和筛选。根据机械强度的差异将仁和壳部分分离。通过高光谱荧光监测，从冷榨亚麻籽饼中分离出的富含 LO 的籽粒部分进一步用作 LO 生产的原料。经过乙醇提取、分配、沉淀，从冷榨亚麻籽饼中提取出LOs，纯度为91.4%。该方法可以作为可行的亚麻籽饼增值策略，并能够制备其他极性化合物，例如亚麻木脂素和粘液。
更新日期：2024-04-09
中文翻译：
干法筛选亚麻籽饼制备环亚麻肽（linusorb）的两步法
Linusorbs (LO) 是一种环亚麻肽，是一组环状疏水性肽，由于具有多种健康益处，被认为是亚麻籽油的有价值的副产品。目前应用的丙酮或甲醇提取可能会污染进一步食品级应用的原料。本研究以亚麻籽饼为原料，建立了一种从压榨饼中制备亚麻籽油的实用方法。首先，对15个亚麻籽品种的LO组成进行了分析。接下来，将冷榨饼进行机械研磨和筛选。根据机械强度的差异将仁和壳部分分离。通过高光谱荧光监测，从冷榨亚麻籽饼中分离出的富含 LO 的籽粒部分进一步用作 LO 生产的原料。经过乙醇提取、分配、沉淀，从冷榨亚麻籽饼中提取出LOs，纯度为91.4%。该方法可以作为可行的亚麻籽饼增值策略，并能够制备其他极性化合物，例如亚麻木脂素和粘液。
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