Functional Characterization of Sesquiterpene Synthases and P450 Enzymes in Flammulina velutipes for Biosynthesis of Spiro [4.5] Decane Terpene,Journal of Agricultural and Food Chemistry

当前位置： X-MOL 学术 › J. Agric. Food Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Functional Characterization of Sesquiterpene Synthases and P450 Enzymes in Flammulina velutipes for Biosynthesis of Spiro [4.5] Decane Terpene
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-04-12 , DOI: 10.1021/acs.jafc.4c01636
Haiyan Han ₁ , Ping Zhang ₁ , Zhekai Xie ₂ , Jianzhao Qi _{1,

3} , Pengchao Wang ₁ , Chang Li ₄ , Zheyong Xue ₁ , Ruibo Wu ₂ , Chengwei Liu ₁

Affiliation

Flammulina velutipes, a popular edible mushroom, contains sesquiterpenes with potential health benefits. We characterized 12 sesquiterpene synthases and one P450 enzyme in F. velutipes using Aspergillus oryzae as a heterologous expression system, culminating in the biosynthesis of 16 distinct sesquiterpene compounds. An enzyme encoded by the axeB gene responsible for the synthesis of the spiro [4.5] decane compound axenol was discovered, and the mechanism of spirocycle formation was elucidated through quantum mechanical calculations. Furthermore, we delineated the role of a P450 enzyme colocated with AxeB in producing the novel compound 3-oxo-axenol. Our findings highlight the diverse array of sesquiterpene skeletons and functional groups biosynthesized by these enzymes in F. velutipes and underscore the effectiveness of the A. oryzae system as a heterologous host for expressing genes in the Basidiomycota genome. These insights into the biosynthesis of bioactive compounds in F. velutipes have significant implications for functional food and drug development.

中文翻译：

金针菇中倍半萜合成酶和 P450 酶用于螺[4.5]癸烷萜生物合成的功能表征

金针菇是一种流行的食用蘑菇，含有具有潜在健康益处的倍半萜烯。我们使用米曲霉作为异源表达系统，对F. velutipes中的 12 种倍半萜合酶和一种 P450 酶进行了表征，最终生物合成了 16 种不同的倍半萜化合物。发现了axeB基因编码的负责合成螺[4.5]癸烷化合物axenol的酶，并通过量子力学计算阐明了螺环形成机制。此外，我们描述了与 AxeB 共置的 P450 酶在生产新型化合物 3-oxo-axenol 中的作用。我们的研究结果强调了F. velutipes中这些酶生物合成的多种倍半萜骨架和功能基团，并强调了米曲霉系统作为表达担子菌门基因组中基因的异源宿主的有效性。这些对F. velutipes生物活性化合物生物合成的见解对于功能性食品和药物开发具有重要意义。

更新日期：2024-04-12

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南