The tumor immune microenvironment has manifested a crucial correlation with tumor occurrence, development, recurrence, and metastasis. To explore the mechanisms intrinsic to osteosarcoma (OS) initiation and progression, this study synthesizes multiple single‐cell RNA sequencing data sets, constructing a comprehensive landscape of the OS microenvironment. Integrating single‐cell RNA sequencing with bulk RNA sequencing data has enabled the identification of a significant correlation between heightened expression of the fatty acid metabolism‐associated gene (C1QBP) and patient survival in OS. C1QBP not only amplifies the proliferation, migration, invasion, and anti‐apoptotic properties of OS but also instigates cisplatin resistance. Subsequent investigations suggest that C1QBP potentially promotes macrophage polarization from monocytes/macrophages toward M2 and M3 phenotypes. Consequently, C1QBP may emerge as a novel target for modulating OS progression and resistance therapy.

中文翻译：

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通过单细胞多维分析破译骨肉瘤免疫微环境重塑和C1QBP驱动的耐药性的时空进化过程

肿瘤免疫微环境与肿瘤的发生、发展、复发和转移有着至关重要的相关性。为了探索骨肉瘤 (OS) 发生和进展的内在机制，本研究综合了多个单细胞 RNA 测序数据集，构建了 OS 微环境的全面景观。将单细胞 RNA 测序与批量 RNA 测序数据相结合，能够识别脂肪酸代谢相关基因的增强表达之间的显着相关性（C1QBP）和 OS 中的患者生存率。 C1QBP 不仅增强 OS 的增殖、迁移、侵袭和抗凋亡特性，而且还会引发顺铂耐药。随后的研究表明，C1QBP 可能促进巨噬细胞从单核细胞/巨噬细胞向 M2 和 M3 表型的极化。因此，C1QBP 可能成为调节 OS 进展和耐药治疗的新靶点。