<br>植物乳杆菌衍生的 Indole-3-乳酸通过 AhR/Nrf2/NF-κB 轴改善肠道屏障完整性,Journal of Agricultural and Food Chemistry

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植物乳杆菌衍生的 Indole-3-乳酸通过 AhR/Nrf2/NF-κB 轴改善肠道屏障完整性
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-04-10 , DOI: 10.1021/acs.jafc.4c01622
Arong Wang _{1,

2} , Cheng Guan _{1,

2} , Tieqi Wang _{1,

2} , Guangqing Mu _{1,

2} , Yanfeng Tuo _{1,

2}

Affiliation

我们之前的研究表明，植物乳杆菌DPUL-S164衍生的吲哚-3-乳酸（ILA）通过激活芳烃受体（AhR）和核因子红细胞2相关因子2（Nrf2）信号通路改善肠上皮细胞屏障损伤并促进紧密连接蛋白的表达。本研究通过右旋糖酐硫酸钠诱导的溃疡性结肠炎小鼠模型，进一步探讨了植物乳杆菌DPUL-S164在减轻小鼠肠道屏障损伤方面的关键物质。与死植物乳杆菌DPUL-S164 (D-S164) 相比，活植物乳杆菌DPUL-S164 (S164) 及其色氨酸代谢物 ILA 对抗生素混合物和钠治疗小鼠的肠道屏障损伤显示出有效的改善作用硫酸葡聚糖，表明植物乳杆菌DPUL-S164改善肠道屏障损伤的关键物质是其细胞外代谢物。此外，S164 及其色氨酸代谢物 ILA 通过激活 AhR-Nrf2 通路和抑制核因子 kappa-B (NF-κB) 通路，改善肠道屏障损伤并抑制肠道炎症。这些结果表明，植物乳杆菌DPUL-S164 主要通过产生 ILA 作为配体来激活 AhR 途径，从而改善小鼠肠上皮屏障损伤。

"点击查看英文标题和摘要"

Lactiplantibacillus plantarum-Derived Indole-3-lactic Acid Ameliorates Intestinal Barrier Integrity through the AhR/Nrf2/NF-κB Axis

Our previous studies have shown that Lactiplantibacillus plantarum DPUL-S164-derived indole-3-lactic acid (ILA) ameliorates intestinal epithelial cell barrier injury by activating aryl hydrocarbon receptor (AhR) and nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathways and promoting tight junction protein expression. This study further explored the crucial substances of L. plantarum DPUL-S164 in alleviating intestinal barrier damage in mice through a dextran sodium sulfate-induced ulcerative colitis mouse model. Compared to dead L. plantarum DPUL-S164 (D-S164), live L. plantarum DPUL-S164 (S164) and its tryptophan metabolite, ILA, showed an effective ameliorating effect on the intestinal barrier injury of mice treated by antibiotic cocktail and sodium dextran sulfate, suggesting that the crucial substances of L. plantarum DPUL-S164 ameliorating intestinal barrier injury are its extracellular metabolites. Furthermore, S164 and its tryptophan metabolite, ILA, ameliorate intestinal barrier injury and suppress intestinal inflammation by activating the AhR–Nrf2 pathway and inhibiting the nuclear factor kappa-B (NF-κB) pathway. These results suggest that L. plantarum DPUL-S164 ameliorates intestinal epithelial barrier damage in mice, primarily by producing ILA as a ligand to activate the AhR pathway.

更新日期：2024-04-10

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