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Microneedle System with Biomarker-Activatable Chromophore as Both Optical Imaging Probe and Anti-bacterial Agent for Combination Therapy of Bacterial-Infected Wounds and Outcome Monitoring
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2024-04-09 , DOI: 10.1021/acsami.4c03534 Juan Ouyang 1 , Lihe Sun 1 , Zunpan She 1 , Rong Li 1 , Fang Zeng 1 , Zhicheng Yao 2 , Shuizhu Wu 1
ACS Applied Materials & Interfaces ( IF 8.3 ) Pub Date : 2024-04-09 , DOI: 10.1021/acsami.4c03534 Juan Ouyang 1 , Lihe Sun 1 , Zunpan She 1 , Rong Li 1 , Fang Zeng 1 , Zhicheng Yao 2 , Shuizhu Wu 1
Affiliation
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Wounds infected with bacteria, if left untreated, have the potential to escalate into life-threatening conditions, such as sepsis, which is characterized by widespread inflammation and organ damage. A comprehensive approach to treating bacterial-infected wounds, encompassing the control of bacterial infection, biofilm eradication, and inflammation regulation, holds significant importance. Herein, a microneedle (MN) patch (FM@ST MN) has been developed, with silk fibroin (SF) and tannic acid-based hydrogel serving as the matrix. Encapsulated within the MNs are the AIEgen-based activatable probe (FQ-H2O2) and the NLRP3 inhibitor MCC950, serving as the optical reporter/antibacterial agent and the inflammation regulator, respectively. When applied onto bacterial-infected wounds, the MNs in FM@ST MN penetrate bacterial biofilms and gradually degrade, releasing FQ-H2O2 and MCC950. The released FQ-H2O2 responds to endogenously overexpressed reactive oxygen species (H2O2) at the wound site, generating a chromophore FQ-OH which emits noticeable NIR-II fluorescence and optoacoustic signals, enabling real-time imaging for outcome monitoring; and this chromophore also exhibits potent antibacterial capability due to its dual positive charges and shows negligible antibacterial resistance. However, the NLRP3 inhibitor MCC950, upon release, suppresses the activation of NLRP3 inflammasomes, thereby mitigating the inflammation triggered by bacterial infections and facilitating wound healing. Furthermore, SF in FM@ST MN aids in tissue repair and regeneration by promoting the proliferation of epidermal cells and fibroblasts and collagen synthesis. This MN system, free from antibiotics, holds promise as a solution for treating and monitoring bacterially infected wounds without the associated risk of antimicrobial resistance.
中文翻译：
具有生物标记可激活发色团作为光学成像探针和抗菌剂的微针系统，用于细菌感染伤口的联合治疗和结果监测
感染细菌的伤口如果不及时治疗，有可能升级为危及生命的病症，例如败血症，其特点是广泛的炎症和器官损伤。治疗细菌感染伤口的综合方法，包括控制细菌感染、根除生物膜和炎症调节，具有重要意义。在此，开发了一种微针（MN）贴片（ FM@ST MN ），以丝素蛋白（SF）和单宁酸基水凝胶作为基质。 MN 内封装有基于 AIEgen 的可激活探针 ( FQ-H 2 O 2 ) 和NLRP3抑制剂 MCC950，分别充当光学报告分子/抗菌剂和炎症调节剂。当应用于细菌感染的伤口时， FM@ST MN中的MN穿透细菌生物膜并逐渐降解，释放FQ-H 2 O 2和MCC950。释放的FQ-H 2 O 2响应伤口部位内源性过度表达的活性氧 (H 2 O 2 )，生成发色团 FQ-OH，发出明显的 NIR-II 荧光和光声信号，从而实现结果的实时成像监控；而且这种发色团还由于其双正电荷而表现出强大的抗菌能力，并且显示出可忽略不计的抗菌耐药性。 然而， NLRP3抑制剂MCC950释放后会抑制NLRP3炎症小体的激活，从而减轻细菌感染引发的炎症并促进伤口愈合。此外， FM@ST MN中的 SF 通过促进表皮细胞和成纤维细胞的增殖以及胶原蛋白的合成来帮助组织修复和再生。这种 MN 系统不含抗生素，有望成为治疗和监测细菌感染伤口的解决方案，且不存在相关的抗菌药物耐药性风险。
更新日期：2024-04-09
中文翻译：
具有生物标记可激活发色团作为光学成像探针和抗菌剂的微针系统，用于细菌感染伤口的联合治疗和结果监测
感染细菌的伤口如果不及时治疗，有可能升级为危及生命的病症，例如败血症，其特点是广泛的炎症和器官损伤。治疗细菌感染伤口的综合方法，包括控制细菌感染、根除生物膜和炎症调节，具有重要意义。在此，开发了一种微针（MN）贴片（ FM@ST MN ），以丝素蛋白（SF）和单宁酸基水凝胶作为基质。 MN 内封装有基于 AIEgen 的可激活探针 ( FQ-H 2 O 2 ) 和NLRP3抑制剂 MCC950，分别充当光学报告分子/抗菌剂和炎症调节剂。当应用于细菌感染的伤口时， FM@ST MN中的MN穿透细菌生物膜并逐渐降解，释放FQ-H 2 O 2和MCC950。释放的FQ-H 2 O 2响应伤口部位内源性过度表达的活性氧 (H 2 O 2 )，生成发色团 FQ-OH，发出明显的 NIR-II 荧光和光声信号，从而实现结果的实时成像监控；而且这种发色团还由于其双正电荷而表现出强大的抗菌能力，并且显示出可忽略不计的抗菌耐药性。 然而， NLRP3抑制剂MCC950释放后会抑制NLRP3炎症小体的激活，从而减轻细菌感染引发的炎症并促进伤口愈合。此外， FM@ST MN中的 SF 通过促进表皮细胞和成纤维细胞的增殖以及胶原蛋白的合成来帮助组织修复和再生。这种 MN 系统不含抗生素，有望成为治疗和监测细菌感染伤口的解决方案，且不存在相关的抗菌药物耐药性风险。
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