<br>化学和生物材料的扫频拉曼光谱,Journal of Biomedical Optics

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化学和生物材料的扫频拉曼光谱
Journal of Biomedical Optics ( IF 3.0 ) Pub Date : 2024-04-01 , DOI: 10.1117/1.jbo.29.s2.s22703
Jeonggeun Song _{1,

2} , Peter T C So ₂ , Hongki Yoo ₁ , Jeon Woong Kang ₂

Affiliation

意义拉曼光谱已被用作化学分析的强大工具，能够从各种样品中无创地获取分子指纹。拉曼光谱已被证明在许多领域都很有价值，包括制药、材料科学和生物医学。目前正在进行积极的研究和开发工作，以便将该分析仪器引入现场，从而使原位拉曼测量能够应用于更广泛的应用。使用固定窄带源的色散拉曼光谱是获取拉曼光谱的常用方法。然而，色散拉曼光谱需要庞大的光谱仪，这限制了其现场适用性。因此，迫切需要开发一种便携式、灵敏的拉曼系统。目的我们开发了一种紧凑型扫频拉曼（SS-Raman）光谱系统，并提出了一种信号处理方法来减轻硬件限制。我们通过从化学和生物样品中获取拉曼光谱来展示 SS-拉曼光谱的功能。然后将这些光谱与使用传统色散拉曼光谱系统获得的拉曼光谱进行比较。方法SS-拉曼光谱系统使用波长扫描源激光器（822 至 842 nm）、带宽为 1.5 nm 的带通滤波器和低-噪声硅光接收器。拉曼光谱是从各种化学样品中获取的，包括苯丙氨酸、羟基磷灰石、葡萄糖和对乙酰氨基酚。通过计算SS-拉曼光谱与传统系统光谱之间的相关系数，与传统色散拉曼光谱进行比较分析。此外，从猪组织的横截面获得拉曼图，证明了SS-拉曼光谱在生物样品中的适用性。结果我们开发了一个紧凑的SS-拉曼系统，并通过从化学和生物材料中获取拉曼光谱来验证其性能。我们简单的信号处理方法提高了拉曼光谱的质量，而不会产生高昂的成本。苯丙氨酸、羟基磷灰石、葡萄糖和对乙酰氨基酚的拉曼光谱在 900 至 1200 cm−1 范围内观察到。与色散拉曼光谱获得的结果相比，结果得到验证，相关系数分别为 0.88、0.84、0.87 和 0.73。此外，我们对猪组织的横截面进行了扫描，以生成生物组织映射图，提供有关猪组织成分的信息。结论我们通过获得化学和化学物质的拉曼光谱，展示了所提出的紧凑型 SS-拉曼光谱系统的功能。生物材料，利用简单的信号处理。我们预计SS-拉曼光谱将应用于各个领域，包括生物医学和化学应用。

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Swept-source Raman spectroscopy of chemical and biological materials

SignificanceRaman spectroscopy has been used as a powerful tool for chemical analysis, enabling the noninvasive acquisition of molecular fingerprints from various samples. Raman spectroscopy has proven to be valuable in numerous fields, including pharmaceutical, materials science, and biomedicine. Active research and development efforts are currently underway to bring this analytical instrument into the field, enabling in situ Raman measurements for a wider range of applications. Dispersive Raman spectroscopy using a fixed, narrowband source is a common method for acquiring Raman spectra. However, dispersive Raman spectroscopy requires a bulky spectrometer, which limits its field applicability. Therefore, there has been a tremendous need to develop a portable and sensitive Raman system.AimWe developed a compact swept-source Raman (SS-Raman) spectroscopy system and proposed a signal processing method to mitigate hardware limitations. We demonstrated the capabilities of the SS-Raman spectroscopy by acquiring Raman spectra from both chemical and biological samples. These spectra were then compared with Raman spectra obtained using a conventional dispersive Raman spectroscopy system.ApproachThe SS-Raman spectroscopy system used a wavelength-swept source laser (822 to 842 nm), a bandpass filter with a bandwidth of 1.5 nm, and a low-noise silicon photoreceiver. Raman spectra were acquired from various chemical samples, including phenylalanine, hydroxyapatite, glucose, and acetaminophen. A comparative analysis with the conventional dispersive Raman spectroscopy was conducted by calculating the correlation coefficients between the spectra from the SS-Raman spectroscopy and those from the conventional system. Furthermore, Raman mapping was obtained from cross-sections of swine tissue, demonstrating the applicability of the SS-Raman spectroscopy in biological samples.ResultsWe developed a compact SS-Raman system and validated its performance by acquiring Raman spectra from both chemical and biological materials. Our straightforward signal processing method enhanced the quality of the Raman spectra without incurring high costs. Raman spectra in the range of 900 to 1200 cm−1 were observed for phenylalanine, hydroxyapatite, glucose, and acetaminophen. The results were validated with correlation coefficients of 0.88, 0.84, 0.87, and 0.73, respectively, compared with those obtained from dispersive Raman spectroscopy. Furthermore, we performed scans across the cross-section of swine tissue to generate a biological tissue mapping plot, providing information about the composition of swine tissue.ConclusionsWe demonstrate the capabilities of the proposed compact SS-Raman spectroscopy system by obtaining Raman spectra of chemical and biological materials, utilizing straightforward signal processing. We anticipate that the SS-Raman spectroscopy will be utilized in various fields, including biomedical and chemical applications.

更新日期：2024-04-01

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