Collagen/chitosan/genipin hydrogel loaded with phycocyanin nanoparticles and ND-336 for diabetic wound healing,International Journal of Biological Macromolecules

当前位置： X-MOL 学术 › Int. J. Biol. Macromol. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Collagen/chitosan/genipin hydrogel loaded with phycocyanin nanoparticles and ND-336 for diabetic wound healing
International Journal of Biological Macromolecules ( IF 7.7 ) Pub Date : 2024-03-28 , DOI: 10.1016/j.ijbiomac.2024.131220
Zhiye Li ₁ , Chenyao Qian ₁ , Xiaodan Zheng ₁ , Xueyong Qi ₁ , Jingyue Bi ₁ , Huan Wang ₁ , Jin Cao ₁

Affiliation

Diabetic wound healing remains a healthcare challenge due to the overexpression of matrix metalloproteinase-9 (MMP-9) and the imbalance between angiogenic factors and vascular inhibitory factors. In this study, we developed a nanocomposite injectable collagen/chitosan hydrogel for the treatment of delayed diabetic wound healing, which can promote cell migration to the wound site (through the addition of phycocyanin) and reduce the expression of MMP-9 (through the use of ND-336) to improve the therapeutic effect of diabetic wound healing. Furthermore, different weight ratios of collagen and chitosan hydrogels were prepared to select the hydrogel with proper mechanical properties. In vitro experiments confirmed that all hydrogels have favorable biocompatibility and hemocompatibility. Notably, Gel 2, with a weight ratio of collagen and chitosan at 25:75, was found to have an excellent capability to facilitate cell migration and in vivo studies further proved that Gel 2 nanocomposite hydrogel had the best ability to improve diabetic wound healing by promoting cell migration and decreasing MMP-9 expression. The collagen/chitosan/genipin hydrogel loaded phycocyanin and ND-336 can be harnessed for non-toxic and efficient treatment of wound healing management of diabetes.

中文翻译：

胶原/壳聚糖/京尼平水凝胶负载藻蓝蛋白纳米颗粒和 ND-336 用于糖尿病伤口愈合

由于基质金属蛋白酶 9 (MMP-9) 的过度表达以及血管生成因子和血管抑制因子之间的不平衡，糖尿病伤口愈合仍然是一个医疗挑战。在这项研究中，我们开发了一种纳米复合材料可注射胶原/壳聚糖水凝胶，用于治疗糖尿病伤口愈合延迟，它可以促进细胞迁移到伤口部位（通过添加藻蓝蛋白）并减少MMP-9的表达（通过使用ND-336）提高糖尿病伤口愈合的治疗效果。此外，制备了不同重量比的胶原蛋白和壳聚糖水凝胶，以选择具有合适机械性能的水凝胶。体外实验证实所有水凝胶均具有良好的生物相容性和血液相容性。值得注意的是，胶原蛋白和壳聚糖重量比为25:75的Gel 2被发现具有优异的促进细胞迁移的能力，体内研究进一步证明Gel 2纳米复合水凝胶具有最佳的改善糖尿病伤口愈合的能力。促进细胞迁移并降低 MMP-9 表达。负载藻蓝蛋白和ND-336的胶原蛋白/壳聚糖/京尼平水凝胶可用于糖尿病伤口愈合管理的无毒且有效的治疗。

更新日期：2024-03-28

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南