Dual-Activated H2O2-Responsive AIE Probes for Oocyte Quality Assessment,Analytical Chemistry

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Dual-Activated H2O2-Responsive AIE Probes for Oocyte Quality Assessment
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-04-06 , DOI: 10.1021/acs.analchem.4c00160
Xiyuan Dong ₁ , Hanzhi Ouyang ₂ , Xiaoding Lou ₂ , Fan Xia ₂ , Lei Jin ₁ , Shixuan Wang ₁ , Jun Dai ₁

Affiliation

Nonobstructive azoospermia (NOA) is an important cause of infertility, and intracytoplasmic sperm injection (ICSI) is the mainstay of treatment for these patients. In cases where a sufficient number of sperm (usually 1–2) is not available, the selection of oocytes for ICSI is a difficult problem that must be solved. Here, we constructed a dual-activated oxidative stress-responsive AIE probe, b-PyTPA. The strong donor–acceptor configuration of b-PyTPA leads to twisted intramolecular charge transfer (TICT) effect that quenches the fluorescence of the probe, however, H₂O₂ would specifically remove the boronatebenzyl unit and release a much weaker acceptor, which inhibits TICT and restores the fluorescence. In addition, the presence of a pyridine salt makes b-PyTPA more hydrophilic, whereas removal of the pyridine salt increases the hydrophobicity of PyTPA, which triggers aggregation and further enhances fluorescence. Thus, the higher the intracellular level of oxidative stress, the stronger the fluorescence. In vitro, this dual-activated fluorescent probe is capable of accurately detecting senescent cells (high oxidative stress). More importantly, b-PyTPA was able to characterize senescent oocytes, as assessed by the level of oxidative stress. It is also possible to identify high quality oocytes from those obtained for subsequent ICSI. In conclusion, this dual-activated oxidative stress-assessment probe enables the quality assessment of oocytes and has potential application in ICSI.

中文翻译：

用于卵母细胞质量评估的双激活 H2O2 响应 AIE 探针

非梗阻性无精子症（NOA）是不孕的重要原因，卵胞浆内单精子注射（ICSI）是这些患者的主要治疗方法。在无法获得足够数量的精子（通常为1-2个）的情况下，选择用于ICSI的卵母细胞是必须解决的难题。在这里，我们构建了一种双激活氧化应激响应 AIE 探针 b-PyTPA。 b-PyTPA 的强供体-受体构型会导致扭曲分子内电荷转移 (TICT) 效应，从而猝灭探针的荧光，然而，H ₂ O ₂会特异性去除硼酸苄基单元并释放出较弱的受体，从而抑制 TICT并恢复荧光。此外，吡啶盐的存在使b-PyTPA更具亲水性，而吡啶盐的去除增加了PyTPA的疏水性，从而引发聚集并进一步增强荧光。因此，细胞内氧化应激水平越高，荧光越强。在体外，这种双激活荧光探针能够准确检测衰老细胞（高氧化应激）。更重要的是，b-PyTPA 能够通过氧化应激水平来表征衰老卵母细胞的特征。还可以从随后 ICSI 获得的卵母细胞中鉴定出高质量的卵母细胞。总之，这种双激活氧化应激评估探针能够评估卵母细胞的质量，并在 ICSI 中具有潜在的应用。

更新日期：2024-04-06

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