The absence of N₂ flux measurements in liquid manure-amended soils has resulted in a poor understanding of the effect of manure application on gaseous N losses. The aim of this study was to quantify N₂, N₂O, CO₂, N₂O reduction to N₂, depth distribution of moisture, water-extractable organic C, NO₃⁻, NH₄⁺, pH, and diffusivity in a laboratory incubation experiment with an arable silt-loam soil. To quantify N processes and gaseous fluxes, ¹⁵N tracing was applied. An artificial livestock slurry-mixture was added to the soil in various treatments (control, surface or injected application; slurry-application rate: 42.9 kg N ha^− 1; soil water content of either 40% or 60% water-filled pore space (WFPS)). The soil was incubated for 10 days. The depth distribution of the control parameters was measured twice during the experiment on days 5 and 10. The average increase in N₂ and N₂O fluxes from denitrification was about 900% in slurry-amended soils. The highest N₂ and N₂O fluxes from denitrification were measured in the slurry injection, 60% WFPS treatment (7.83 ± 3.50 and 11.22 ± 7.60 mg N m^− 2 d^− 1, respectively). The hypothesis that injected slurry at a higher water content enhances denitrification was confirmed. This study provides important insights into the formation, spatial and temporal variation of the manure-soil hotspot and its impact on the denitrification process. The results will form part of a dataset to develop, improve and test manure application submodules of biogeochemical models and will help to understand in detail the effect of hotspots on N-cycling in manure-treated soils.

由于缺乏液体肥料改良土壤中的 N ₂通量测量，导致人们对肥料施用对气态氮损失的影响了解甚少。本研究的目的是量化 N ₂、N ₂ O、CO ₂、N ₂ O 还原为 N ₂、水分深度分布、水可萃取有机 C、NO ₃ ⁻、NH ₄ ⁺、pH 和扩散率用可耕种的粉砂壤土进行实验室孵化实验。为了量化 N 过程和气体通量，应用了¹⁵ N 示踪。在各种处理中将人工牲畜泥浆混合物添加到土壤中（对照、表面或注射施用；泥浆施用量：42.9 kg N ha ^{− 1}；土壤含水量为 40% 或 60% 充水孔隙空间（世界粮食计划署））。将土壤培养10天。在第 5 天和第 10 天的实验过程中，对控制参数的深度分布进行了两次测量。在泥浆改良土壤中，反硝化过程中N ₂和 N ₂ O 通量的平均增加量约为 900%。在浆液注入、60% WFPS 处理中测量了反硝化产生的最高 N ₂和 N _{2 O 通量（分别为 7.83 ± 3.50 和 11.22 ± 7.60 mg N m} ^{− 2} d ^{− 1}）。证实了注入较高含水量的浆料可增强反硝化作用的假设。这项研究为粪便-土壤热点的形成、时空变化及其对反硝化过程的影响提供了重要的见解。研究结果将构成数据集的一部分，用于开发、改进和测试生物地球化学模型的粪便施用子模块，并将有助于详细了解热点对粪便处理土壤中氮循环的影响。